论文部分内容阅读
目的 检测血清中乙型肝炎病毒(HBV)DNA并进行基因分型。方法 采用聚合酶链反应(PCR)扩增HBV DNA后,用不同的探针,利用微板杂交法将其分为6个亚型。结果 150例HBV DNA阳性病人中,B型50例、C型36例、D型13例、F型3例和A型1例,没有发现E型。另有47例为混合型,其中以B、D型(18例)、C、D型(20例)为主,分别占混合型的38.30％与42.6％。受检病人中,B型感染者丙氨酸氨基转移酶水平明显增高;B型感染者比C型感染者抗HBe阳性率高,分别为64.0％和30.5％,而C型感染者比B型感染者乙型肝炎e抗原阳性率高,分别为47.2％和21.0％;年龄、性别与基因型关系不明显。结论 深圳地区BBV基因亚型以B型为主,C型次之,其他较少。基因型与肝炎程度有一定关系。
Objective To detect hepatitis B virus (HBV) DNA in serum and genotype it. Methods The HBV DNA was amplified by polymerase chain reaction (PCR) and then divided into 6 subtypes using different probes by using microplate hybridization. Results Among 150 cases of HBV DNA positive patients, 50 cases were type B, 36 cases were type C, 13 cases were type D, 3 cases were type F and 1 case was type A. No type E was found. Another 47 cases were mixed, of which B, D type (18 cases), C, D type (20 cases), accounting for 38.30% and 42.6% of mixed type. Among the tested patients, the level of alanine aminotransferase was significantly increased in patients with type B infection; the positive rate of anti-HBe in type B patients was 64.0% and 30.5% higher than those in type C ones, while those in type C patients were higher than those in type B patients The positive rate of hepatitis B e antigen in infected persons was 47.2% and 21.0% respectively. The age, sex and genotype were not obvious. Conclusion BBV gene subtype in Shenzhen is mainly B type, followed by C type, while others are less. Genotype and degree of hepatitis have a certain relationship.