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目的：研究ＶＥＧＦ，ｐ５３和ｐ２１ｒａｓ在原发性肝细胞癌（ＨＣＣ）的表达和三者间的相关性及其与ＨＣＣ病理分级，转移行为的关系。方法：应用免疫组织化学Ｓ－Ｐ法对３４例ＨＣＣ的癌组织中的ＶＥＧＦ，ｐ２１ｒａｓ和ｐ５３的表达作了研究。结果：ＶＥＧＦ，ｐ２１ｒａｓ和ｐ５３三者在肝癌和癌旁组织的阳性率均有非常显著的差异（Ｐ＜０．０１），ＶＥＧＦ的表达与ｐ２１ｒａｓ和ｐ５３均呈显著的正相关（Ｐ＜０．０１）。三者间的协同表达亦与ＨＣＣ的病理分级和转移行为有关，而与肿瘤大小，ＡＦＰ水平，ＨＢｓＡｇ阳性无关。结论：ＶＥＧＦ可能在ＨＣＣ的形成，发展和转移中与ｐ５３和ｒａｓ基因协同作用，三者的协同作用可能与ＨＣＣ的病理分级和转移行为有关。
OBJECTIVE: To study the expression of VEGF, p53 and p21ras in primary hepatocellular carcinoma (HCC) and their correlation with pathological grading and metastatic behavior of HCC. Methods: The expression of VEGF, p21ras and p53 in 34 cases of HCC were studied by immunohistochemical S-P method. Results: The positive rates of VEGF, p21ras and p53 in hepatocellular carcinoma and paracancerous tissues were significantly different (P<0.01). There was a significant positive correlation between the expression of VEGF and p21ras and p53 (P<0. 01). The synergistic expression among the three was also related to pathological grade and metastatic behavior of HCC, but not to tumor size, AFP level, HBsAg positive. Conclusion: VEGF may act synergistically with p53 and ras genes in the formation, development and metastasis of HCC. The synergistic effect of these three factors may be related to the pathological grade and metastatic behavior of HCC.