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Objective:To characterizeVP4, VP6, VP7andNSP4 genes of representativeGBR strains (NIV-005625, NIV-04622 andNIV-094456) detected as the major etiologic agent in the outbreaks of gastroenteritis in west India.Methods: Fecal specimens collected during the outbreaks of gastroenteritis were processed forRNA isolation,RT-PCR usingGBR VP4, VP6, VP7andNSP4 gene specific primers, nucleotide sequencing of the amplicons and phylogenetic analysis of the sequences.Results:Phylogenetic analysis of all of theVP4, VP6, VP7 andNSP4 gene sequences revealed clustering ofGBR strains in Indian-Bangladeshi lineage of genotypeG2 with95.8%-99.4%nucleotide and97.3%-100.0%amino acid identities. However, all three strains showed the presence of unique amino acid substitutions in theVP4 protein suggesting alteration in the antigenicity of outbreak strains ofGBR. TheVP8*andVP5* regions ofVP4 proteins showed respectively0.5%-6.3%and 0.2%-1.1% amino acid divergence from humanGBR strains of Indian-Bangladeshi lineage.Conclusions:These data confirm the reported variability ofVP8*region and suggest the possible role of this region in the perpetuation ofGBR infections in the environment. This is the first study to document the phylogenetic relationship ofVP4, VP6, VP7 andNSP4 genes of GBRstrains detected in the outbreaks of gastroenteritis from India with the GBR strains from other parts of world.