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目的了解重组人生长激素(rhGH)在体外对人胃、结肠癌细胞有无促进增殖作用。方法实验分对照组、rhGH组、奥沙利铂(L-OHP)组和L-OHP+rhGH组,利用体外细胞培养及流式细胞仪等方法,测定不同浓度的rhGH对人胃癌细胞株MKN45、人结肠癌细胞株Colo320细胞周期和增殖指数(PI)的影响。结果 rhGH在体外不促进MKN45和Colo320细胞分裂增殖。rhGH组与对照组、L-OHP+rhGH组与L-OHP组比较均无统计学意义(P>0.05),L-OHP+rhGH组与对照组比较或L-OHP+rhGH组与对应的rhGH组比较,阻滞于G0～G1期的细胞数增加,S期和G2～M期细胞明显减少,PI明显降低(P<0.01)。结论 rhGH在体外不促进胃、结肠癌细胞的分裂增殖。
Objective To investigate whether recombinant human growth hormone (rhGH) can promote the proliferation of human stomach and colon cancer cells in vitro. Methods The experimental groups were divided into control group, rhGH group, L-OHP group and rhGH group. The effects of different concentrations of rhGH on the proliferation of human gastric cancer cell line MKN45 were determined by in vitro cell culture and flow cytometry , Human colon cancer cell line Colo320 cell cycle and proliferation index (PI). Results rhGH did not promote the proliferation of MKN45 and Colo320 cells in vitro. There was no significant difference between L-OHP + rhGH group and control group, L-OHP + rhGH group and L-OHP group (P> 0.05) Compared with control group, the number of cells arrested in G0 ~ G1 phase increased, the number of cells in S phase and G2 ~ M phase decreased significantly, PI decreased significantly (P <0.01). Conclusion rhGH does not promote the proliferation of gastric and colon cancer cells in vitro.