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目的：研究病毒性心肌炎急性期组织修复性病理改变，探索轻度病毒性心肌炎的法医病理学诊断方法。方法：以适量 Coxsackie B3病毒感染 Balb/c小鼠造成小鼠轻度病毒性心肌炎。利用苦味酸－酸性品红染色法观察病鼠心肌组织胶原纤维的增生情况，并对病鼠及 9例人心肌炎心肌进行 TGF－β 1的 LSAB免疫组化研究。结果：病鼠心肌组织内胶原纤维呈不同程度增生；心肌炎鼠及人心肌炎心肌组织可见大量 TGF－β 1表达； TGF－β 1的表达与胶原纤维的增生呈正相关。结论：病毒性心肌炎急性期即存在组织修复性病理改变， TGF－β 1的阳性表达是反映心肌炎症性损伤修复的灵敏指标。
Objective: To study the histopathological changes of tissue during the acute phase of viral myocarditis and to explore the forensic pathological diagnosis of mild viral myocarditis. Methods: Infection of Balb / c mice with mild Coxsackie B3 virus caused mild viral myocarditis in mice. The proliferation of collagen fibers in myocardium was observed by picric acid-acid fuchsin staining. LSAB immunohistochemistry of TGF-β1 was performed in the myocardium of 9 cases of myocarditis. Results: The collagen fibers in the diseased rat myocardium proliferated in varying degrees. A large number of TGF-β 1 was found in the myocardium of myocarditis and myocarditis; the expression of TGF-β 1 was positively correlated with the proliferation of collagen fibers. CONCLUSION: The acute phase of viral myocarditis exists tissue repair pathological changes. The positive expression of TGF-β 1 is a sensitive indicator of myocardium inflammatory injury.