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目的:研究实验小鼠经卡托普利给药后,小鼠的学习记忆能力的变化及海马区多聚唾液酸神经细胞黏附因子(polysialylated neural cell adhesion molecule,PSA-NCAM)的表达情况。方法:BALB/c小鼠随机分为给药组和对照组,各6只。给药组小鼠腹腔注射卡托普利,每日30 mg/kg,连续7 d;对照组注射等量的无菌生理盐水。给药后对小鼠进行Morris水迷宫试验检测小鼠的学习记忆能力,后取脑切片进行PSA-NCAM免疫组织化学染色。结果:给药组小鼠逃避潜伏期较对照组缩短,而目标象限停留时间及经过平台次数较对照组增加(P<0.05)。免疫组织化学法显示两组小鼠海马区PSA-NCAM表达没有明显差异。结论:对实验小鼠给药卡托普利可促进小鼠学习记忆能力,但该药对小鼠海马区PSA-NCAM表达没有明显作用。
Objective: To investigate the changes of learning and memory ability and the expression of polysialylated neural cell adhesion molecule (PSA-NCAM) in hippocampus of mice after captopril administration. Methods: BALB / c mice were randomly divided into treatment group and control group, 6 in each. The mice in the treatment group were injected intraperitoneally with captopril, 30 mg / kg daily for 7 days. The control group was injected with the same amount of sterile saline. Morris water maze test was used to detect the learning and memory ability of the mice after the administration, and then the brain sections were taken for PSA-NCAM immunohistochemical staining. Results: The escape latency of the mice in the administration group was shorter than that of the control group, while the retention time in the target quadrant and the number of platform passing through the platform were higher than those in the control group (P <0.05). Immunohistochemistry showed no significant difference in the expression of PSA-NCAM in hippocampus between the two groups. Conclusion: Captopril administered to mice can promote learning and memory in mice, but the drug has no obvious effect on the expression of PSA-NCAM in hippocampus of mice.