论文部分内容阅读
为了克隆和研究旋毛虫保护性抗原及特异性诊断抗原基因，我们用异硫氰酸胍从旋毛虫肌肉或幼虫中分离总RNA，用PolyATtractmRNA分离系统统化mRNA，以NotIprimeradapter为引物合成双链cDNA，加SalIadapter,与λgt22A在体外进行连接包装并转染大肠杆菌Y1090r－，构建cDNA文库。结果获得5×105个重组噬菌体，重组率在90％以上．
To clone and study Trichinella spiralis protective antigen and specific diagnostic antigen genes, we isolated total RNA from Trichinella muscle or larvae using guanidinium isothiocyanate, synthesized mRNA using PolyATtract mRNA isolation system, synthesized double-stranded cDNA using NotIprimeradapter as primer , Add SalIadapter, lambda gt22A connected in vitro packaging and transfection E. coli Y1090r-, to construct a cDNA library. The results obtained 5 × 105 recombinant phage, the recombination rate of more than 90%.