论文部分内容阅读
目的探讨分化抑制蛋白-1(Id-1)和基质金属蛋白酶2(MMP-2)在涎腺腺样囊性癌(SACC)中的表达及其与肿瘤侵袭和转移的相关性。方法用免疫组织化学方法检测Id-1和MMP-2在60例SACC组织(实验组)与60例癌旁正常组织(对照组)中的表达情况,分析二者的表达与SACC浸润和侵袭的相关性。结果实验组和对照组的Id-蛋白阳性表达率分别为68.33%(41/60例)和13.33%(8/60例);MMP-2蛋白阳性表达率分别为73.33%(44/60例)和18.33%(11/60例),差异有统计学意义(P<0.001)。Id-1和MMP-2的阳性表达率在有淋巴结转移者分别为83.33%,87.50%、在无淋巴结转移者分别为58.33%,63.90%;在肺转移者分别为88.24%,94.12%、在无肺转移者分别为60.47%,65.12%;在Ⅲ+Ⅳ期者分别为81.48%,88.89%、在Ⅰ+Ⅱ期者分别为57.58%,60.61%;在中/低分化者分别为85.71%,91.42%、在高分化者分别为44.00%,48.00%;在伴脉管浸润者分别为89.47%,100.00%、无脉管浸润者分别为58.54%,60.98%;且Id-1和MMP-2在上述前者的阳性表达率明显高于后者,差异有统计学意义(P<0.05)。Id-1和MMP-2的阳性表达率在男性分别为66.67%,75.00%、在女性分别为70.83%,70.83%;在≥50岁者分别为73.53%,76.47%、在<50岁者分别为61.54%,69.23%;在小涎腺者分别为63.64%,72.73%、在大涎腺者分别为71.05%,73.68%;且Id-1和MMP-2在上述前者和后者的阳性表达率比较差异无统计学意义(P>0.05)。Id-1和MMP-2在SACC中的表达呈正相关。结论 Id-1和MMP-2的异常表达在SACC发展、浸润过程中起重要作用,联合检测Id-1和MMP-2的表达水平有可能作为监测SACC进展和转移的生物学指标。
Objective To investigate the expression of Id-1 and MMP-2 in salivary adenoid cystic carcinoma (SACC) and its relationship with tumor invasion and metastasis. Methods Immunohistochemistry was used to detect the expression of Id-1 and MMP-2 in 60 SACC tissues (experimental group) and 60 adjacent normal tissues (control group). The expressions of Id-1 and MMP-2 in both SACC infiltration and invasion Correlation. Results The positive rates of Id-protein in experimental group and control group were 68.33% (41/60) and 13.33% (8/60), respectively. The positive rates of MMP-2 protein were 73.33% (44/60) And 18.33% (11/60) respectively, the difference was statistically significant (P <0.001). The positive rates of Id-1 and MMP-2 were 83.33% and 87.50% in patients with lymph node metastasis, 58.33% and 63.90% in patients without lymph node metastasis, 88.24% and 94.12% in patients with lung metastasis, respectively Those without metastasis were 60.47% and 65.12% respectively; those in stage Ⅲ + Ⅳ were 81.48% and 88.89% respectively, 57.58% and 60.61% respectively in stage Ⅰ + Ⅱ, and those in stage Ⅰ and Ⅱ were 85.71% , 91.42% in the well-differentiated group, 44.00% in the well-differentiated group and 48.00% in the well-differentiated group, respectively, and 89.47% and 100.00% 2 in the former positive expression rate was significantly higher than the latter, the difference was statistically significant (P <0.05). The positive rates of Id-1 and MMP-2 were 66.67% and 75.00% respectively in males and 70.83% and 70.83% respectively in females and 73.53% and 76.47% in those aged> 50 years, respectively Were 61.54% and 69.23% respectively; those in small salivary glands were 63.64% and 72.73%, respectively, and those in large salivary glands were 71.05% and 73.68% respectively. The positive expressions of Id-1 and MMP-2 in the former and the latter The difference was not statistically significant (P> 0.05). The expression of Id-1 and MMP-2 in SACC were positively correlated. Conclusions The abnormal expression of Id-1 and MMP-2 plays an important role in the development and infiltration of SACC. Combined detection of the expression of Id-1 and MMP-2 may serve as a biological indicator for monitoring the progress and metastasis of SACC.