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目的 :观察 β 七叶皂甙钠对肢体缺血再灌注损伤的保护作用。方法 :用兔造成肢体缺血再灌注损伤动物模型。实验分对照组、缺血再灌注组和 β 七叶皂甙钠组。取血浆测定丙二醛、肌酸磷酸激酶、谷草转氨酶和乳酸脱氢酶含量。取骨骼肌标本测定丙二醛、髓过氧化酶活性、肌细胞线粒体钙含量和组织湿 /干重比值。结果 :缺血再灌注组与对照组比较 ,血浆和骨骼肌的各项生化指标显著增高 (P <0 .0 1) ;使用 β 七叶皂甙钠后 ,血浆及骨骼肌各项测定指标较缺血再灌注组相比明显降低 (P <0 .0 5 ,P <0 .0 1)。结论 :β 七叶皂甙钠可减轻肢体缺血再灌注损伤 ,对骨骼肌有保护作用
Objective: To observe the protective effect of β-aescinate on limb ischemia-reperfusion injury. Methods: Animal models of limb ischemia-reperfusion injury were established. Experimental control group, ischemia-reperfusion group and β-aescinate sodium group. Plasma was taken for determination of malondialdehyde, creatine phosphokinase, aspartate aminotransferase and lactate dehydrogenase. Skeletal muscle specimens were taken for determination of malondialdehyde, myeloperoxidase activity, myocyte mitochondrial calcium content and tissue wet / dry weight ratio. Results: Compared with the control group, the biochemical indexes of plasma and skeletal muscle in ischemia / reperfusion group were significantly increased (P <0.01). The indexes of plasma and skeletal muscle were less than those in control group Blood reperfusion group was significantly lower (P <0.05, P <0.01). Conclusion: β-aescinate can reduce limb ischemia-reperfusion injury and have protective effect on skeletal muscle