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磷酸酶普遍存在于动物的各组织中,对磷酸单酯键具有水解活性,是机体生长代谢、保持内环境稳定和维持机体健康所必需的酶。以镜鲤(Cyprinus carpio L.)全同胞家系190个个体为材料,用992对微卫星(SSR)标记进行基因组扫描,采用区间作图法,对肠组织的碱性磷酸酶和酸性磷酸酶进行了QTL定位分析。QTL检测显示:5个QTL区间与酸性磷酸酶活性相关,其中3个QTL为99%染色体水平显著性,分别位于LG2(CA2049-CA2371)、LG25(HLJ2941-HLJ3946)和LG28(CA2263-HLJ2873),另外两个QTL区间为95%染色体水平显著性,位于LG14(HLJ3275-CA174)和LG14(CA1276-CA2208),解释表形变异率范围为8.1%～38.9%;2个与碱性磷酸酶相关的QTL区间均为95%染色体水平显著性,分别位于LG8和LG13,可解释变异率分别为7.3%和7.0%。
Phosphatase is ubiquitous in animal tissues and has hydrolytic activity on phosphate monoester bonds. It is an enzyme necessary for the growth and metabolism of the body, maintaining the internal environment and maintaining the health of the body. A total of 190 individuals from the entire sib family of Cyprinus carpio L. were used for genome scanning with 992 SSR markers. The interval mapping method was used to detect alkaline phosphatase and acid phosphatase in intestinal tissues QTL mapping analysis. QTL analysis showed that 5 QTL intervals were related to acid phosphatase activity, of which 3 QTLs were significantly (99%) chromosomally located at LG2 (CA2049-CA2371), LG25 (HLJ2941-HLJ3946) and LG28 (CA2263-HLJ2873) The other two QTL intervals were 95% chromosomally significant, located in LG14 (HLJ3275-CA174) and LG14 (CA1276-CA2208), explaining that the variability ranged from 8.1% to 38.9%. Two alkaline phosphatase-related The QTL interval was 95% chromosomally significant, located at LG8 and LG13, respectively, with explained rates of variation of 7.3% and 7.0%, respectively.