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自1983年发现幽门螺杆菌(HP)以来,经学者们10年的研究,已确认该菌是慢性活动性胃炎和消化性溃疡的主要病因,胃的免疫在疾病发生中的作用亦引起了人们的关注,国内这方面的研究很少,所涉及的免疫细胞亦不多.本研究不仅调查了正常和HP~+慢性活动性胃炎胃窦部11种免疫细胞的分布和数量的变化,而且对18例胃粘膜相关淋巴组织(MALT)及淋巴小结(LF)的发生率进行了统计,并对LF内的免疫细胞进行了研究.结果:1.LF的发生率.正常组(HP-6例)偶见淋巴细胞(LC)聚集成堆,这些细胞以T总细胞为主,无一例出现LF.HP~+胃炎组(12例)胃粘膜内可见多处CD_3~+TC聚集成团的现象,7例(58.3%)出现LF.LF的出现与HP的数量有关,HP感染强阳性者(卅)均出现LF,其中2例呈现2～4个LF密集排列.大部分LF位于固有膜的浅层.2.LF的形态及免疫细胞定性研究.所见LF形态各异人F内的LC以 CD_3~+T总细胞为主,CD_3~+细胞主要位于相当于生发中心的周围部分(M区),LF中央(C区)较少.用抗CD_4、CD_8、CD_(57)、CD_(58)和CD_(20)单抗进一步证实,LF内的TC主要是CD_4~+细胞,而CD_3~+细胞主要位于C区,数量极少,在LF内还可见数量较多的NKC、单核细胞和BC.进一步用抗细胞因子单抗检测.发现LF内还有IL-2、IL-8和IFN分泌细胞.结论:HP~+慢性胃炎胃粘膜出现大量MALT,?
Since the discovery of Helicobacter pylori (HP) in 1983, 10 years of research by scholars have confirmed that the bacteria is the main cause of chronic active gastritis and peptic ulcer, and the role of gastric immunity in the disease has also caused people Of the concern, the domestic research in this area is very small, the immune cells involved are not many.This study not only investigated the normal and HP ~ + chronic gastritis 11 kinds of immune cells in the distribution and number of changes in the immune cells, and 18 cases of gastric mucosa-associated lymphoid tissue (MALT) and lymphoid nodules (LF) were statistically analyzed and the immune cells in LF were studied.Results: 1.LF incidence in the normal group (HP-6 cases ) Occasionally, lymphocytes (LC) aggregated into piles, and these cells were mainly T cells. None of them showed the phenomenon of multiple CD_3 ~ + TC aggregating into gastric mass in LF.HP ~ + gastritis group (12 cases) , And the appearance of LF.LF was related to the number of HP in 7 cases (58.3%). LF was found in those with strong HP infection (卅), and 2 to 4 LFs were densely arranged in most of them, most of which were located in the lamina propria Shallow .2.LF morphological and immune cell qualitative study found that LF morphology of different human F within the LC to CD_3 ~ + T cells, CD_3 ~ + cells To be located in the peripheral part (M region) equivalent to the germinal center, there was less LF center (C region) .It was further confirmed by anti-CD_4, CD_8, CD_ (57), CD_ (58) and CD_ (20) Of TCs were mainly CD_4 ~ + cells, while CD_3 ~ + cells were mainly located in C region, with a very small number of NK cells, mononuclear cells and BC in LF, and further detected by anti-cytokine monoclonal antibody. There were IL-2, IL-8 and IFN-secreting cells in LF.Conclusion: There are a large number of MALT in gastric mucosa of HP ~ + chronic gastritis.