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一、概述:色谱法又名色层法,早在1906年为俄国生物学家Цвет所提出。他在填有吸附性物质的玻璃管中,将生物提出液加以过滤,并以溶剂进行冲洗,使原来难以分离的生物色素在玻璃管中得以分离,呈现明显的色带,因而得名“色层”。显然,这种方法仅能用于有色液体;对无色物质则需加显色剂;另外,在柱管的出口处没有鉴定器,不能自动地给出组分含量的信号。当时,色层分离法在分析上的应用并未得到多大发展。直到二次大战前,为研究放射性裂变物质,对离子交换色谱进行了大量研究。在1940年出现了液体分配色谱,
First, an overview: Chromatography, also known as chromatography, as early as 1906 proposed by the Russian biologist Цвет. He filled the adsorbent material in the glass tube, the biological fluid to be filtered and rinsed with a solvent to make the original difficult to separate biological pigment in the glass tube to be separated, showing a clear ribbon, hence the name “color Floor”. Obviously, this method can only be used for colored liquids; for colorless substances it is necessary to add a color reagent; in addition, there is no detector at the outlet of the column tube and the signal of the content of the composition can not be automatically given. At that time, the chromatographic separation method in the analysis of the application has not been much development. Until the Second World War, in order to study the radioactive fission material, ion exchange chromatography has been a lot of research. Liquid chromatography appeared in 1940,