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背景与目的:中枢神经系统血管母细胞(centralnervoussystemhemangioblastoma,CNSHB)的治疗较难,至今病因不明。本研究应用蛋白质组学方法分析HB与正常脑组织的蛋白质表达谱的差异,分离鉴定与HB发病的蛋白质,为研究HB的组织学起源与发病机制提供依据。方法:收集5例血管母细胞瘤与5例正常脑组织标本。提取标本的总蛋白质。通过双向凝胶电泳(2-DE)分离蛋白,确定血管母细胞瘤与正常脑组织的差异表达蛋白质点。应用基质辅助激光解离-飞行时间质谱(MALDI-TOF-MS)鉴定并对鉴定蛋白质进行生物信息学分析。结果:通过双向凝胶电泳,建立了约含600个蛋白质点的血管母细胞瘤及正常脑组织的高分辨率蛋白质表达图谱。两者相比具有较高的同源性。应用ImageMaster2D图像分析软件共发现115个差异蛋白质点。经MALDI-TOF-MS分析后成功鉴定了87个蛋白质点共计47种蛋白质。其中HB组较正常对照组表达上调46个蛋白质点,下调41个蛋白质点。鉴定蛋白质的功能涉及转运、蛋白质折叠与代谢、三羧酸循环、细胞分化、干细胞相关蛋白质等数个方面。对鉴定所得蛋白质Vimentin、14-3-3蛋白进行免疫组化染色可重复本研究的结果。结论:中枢神经系统血管母细胞瘤可能是一种起源于间叶脑组织的肿瘤。其发生是一个多因子参与、多步骤的复杂过程。多种蛋白质如Vimentin及14-3-3蛋白参与了血管母细胞瘤的发病并起到重要的作用。
BACKGROUND & AIM: Central nervous system hemangioblastoma (CNSHB) is more difficult to treat and the cause is unknown. In this study, proteomic profiling of HB and normal brain tissues was used to analyze the differences in protein expression profiles between HB and normal brain tissues. The proteins involved in the pathogenesis of HB were isolated and identified to provide the basis for studying the histological origin and pathogenesis of HB. Methods: Five cases of hemangioblastoma and five normal brain tissues were collected. Extract the total protein of the specimen. Protein was separated by two-dimensional gel electrophoresis (2-DE) to determine differentially expressed protein spots in hemangioblastoma and normal brain tissue. Bioinformatic analysis of the identified proteins was performed using matrix-assisted laser desorption-time of flight mass spectrometry (MALDI-TOF-MS). Results: Two-dimensional gel electrophoresis was used to establish a high-resolution protein expression profile of about 600 protein spots in hemangioblastoma and normal brain tissue. Both have higher homology. A total of 115 differential protein spots were detected using ImageMaster2D image analysis software. A total of 47 proteins were successfully identified by MALDI-TOF-MS analysis at 87 protein spots. In HB group, 46 protein spots were up-regulated and 41 spots were down-regulated compared with normal control group. Identification of protein functions involves several aspects of transport, protein folding and metabolism, tricarboxylic acid cycle, cell differentiation, stem cell-related proteins. The results of this study can be repeated by immunohistochemical staining of the identified protein Vimentin, 14-3-3 protein. Conclusion: Central nervous system hemangioblastoma may be a tumor originated from mesencephalic tissue. Its occurrence is a multi-factor involved, multi-step complex process. A variety of proteins such as Vimentin and 14-3-3 are involved in the pathogenesis of hemangioblastoma and play an important role.