论文部分内容阅读
块菌作为可食用的地下外生菌根真菌,有着重要的经济价值和生态学意义。中国白块菌资源虽然被不断的描述和报道,但形成机制尚未为人所知。前人研究表明,块菌的菌根根际土壤微生物群落对块菌的形成有着重要的影响。因此,本研究以攀枝花块菌(Tuber panzhihuanense)-华山松(Pinus armandii)菌根根际土壤为研究对象,用可培养的方法揭示了其根际土壤的细菌多样性。结果显示,在所分离到的细菌中,β-Proteobacteria占了最大的比例(30.98%),以Burkholderia为优势类群,其次是以Pseudomonas为代表类群的γ-Proteobacteria(28.8%),另外,α-Proteobacteria(14.67%)的主要代表类群为Phyllobacterium和根瘤菌Rhizobium;此外,还分离到了分别以Arthrobacter和Bacillus为优势菌群代表的Actinobacteria(12.5%)和Firmicutes(7.6%);Bacteroidetes中只有唯一的代表菌株Chryseobacterium ureilyticum。另外,就目前对块菌属子实体及其根际土壤内的可培养细菌多样性研究进行了比较和探讨。
As an edible underground ectomycorrhizal fungus, truffle has important economic value and ecological significance. Although the Chinese white truffle resources have been described and reported continuously, the formation mechanism has not been known yet. Previous studies have shown that Truffle rhizosphere soil microbial community has an important impact on the formation of truffles. Therefore, in this study, rhizosphere soils of Tuber panzhihuanense-Pinus armandii were studied, and bacterial diversity in rhizosphere soils was revealed by culturable methods. The results showed that β-Proteobacteria accounted for the largest proportion (30.98%) among the isolates, with Burkholderia as the dominant group, followed by γ-Proteobacteria (28.8%) with Pseudomonas as the representative group, and α- Proteobacteria (14.67%) were mainly Phyllobacterium and Rhizobium. In addition, Actinobacteria (12.5%) and Firmicutes (7.6%), which were dominant strains of Arthrobacter and Bacillus respectively, were isolated. Only the only representatives of Bacteroidetes Strain Chryseobacterium ureilyticum. In addition, the current research on the diversity of culturable bacteria in truffle fruiting body and its rhizosphere soil are compared and discussed.