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目的:研究侧脑室注射甘珀酸后对福尔马林灌胃致内脏疼痛大鼠的延髓迷走孤束复合体内星形胶质细胞和神经元反应的影响。方法:经侧脑室注射缝隙连接阻断剂甘珀酸(carbenoxolone,CBX)后向大鼠胃内灌入2.5%福尔马林2ml诱发内脏疼痛,用免疫组织化学方法观察延髓迷走孤束复合体(VSC)内抗Fos蛋白(标记神经元)和抗胶质原纤维酸性蛋白(标记星形胶质细胞)的单一或双重标记的免疫荧光染色。结果:福尔马林灌胃后大鼠出现烦躁易激惹,呼吸变快,持续1h;而预先侧脑室注射CBX则动物疼痛行为学反应明显减轻。免疫组织化学染色发现福尔马林灌胃后大鼠VSC中的Fos免疫反应数目增强;大鼠预先侧脑室注射CBX后VSC中的Fos免疫反应数目明显减弱。结论:延髓VSC中的星形胶质细胞和神经元参与福尔马林灌胃致内脏痛的调节,星形胶质细胞可能通过缝隙连接影响神经元对内脏痛的调节功能。
AIM: To investigate the effects of intracerebroventricular injection of carbenoxolol on the astrocytes and neurons in the medulla oblongata complex induced by formalin in rats with gut pain. Methods: After intracerebroventricular injection of carboplatin (CBX), a blocker of carbenoxolone (CBX), 2.5 ml formalin in rats was infused into the stomach of stomach to induce visceral pain. Immunohistochemistry was used to observe the expression of medulla oblongata (VSC) with anti-Fos protein (labeled neurons) and anti-glial fibrillary acidic protein (labeled astrocytes) single or double-labeled immunofluorescence staining. Results: After the rats were instilled with formalin, irritable irritability and irritation were found in the rats, which lasted for 1 hour. However, pre-administration of CBX in the lateral ventricle significantly reduced the pain-induced behavior. Immunohistochemical staining showed that the number of Fos immune responses in rat VSCs increased after formalin administration, and the number of Fos immunoreactive cells in VSCs decreased significantly after CBX injection in rats. CONCLUSION: Astrocytes and neurons in medulla oblongata VSC participate in the regulation of visceral pain induced by formalin, and astrocytes may affect neuron regulation of visceral pain through gap junctions.