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肾上腺素具有拟似促黄体生成激素(LH)的作用,可刺激离体大鼠黄体产生cAMP和孕酮。本文作者通过实验进一步论证上述作用并探讨PGF_(2α)抑制CA的机理。作者选用未成熟雌性大鼠,在500～1900小时内控制光照,食物和水随意。诱发排卵和黄体形成:给未成熟雌性大鼠于第28天早晨注射孕血清激素(PMSG)8 IU/0.2 ml盐水以促使滤泡发育,两天后的下午释放内源性的LH/FSH,随之排卵。分离及孵育黄体:将第31、33和35天的大鼠处死、迅速取出卵巢,在立体显微镜下用手分离出黄体并分别定为1、3、5天的黄体。孵育液为改良的Krebs Ringer碳酸氢盐缓冲液,预先在37℃
Epinephrine has a pseudo-luteinizing hormone (LH) effect, can stimulate isolated rat luteal cAMP and progesterone. The authors further demonstrate the above effects through experiments and explore the mechanism of inhibition of CA by PGF_ (2α). The author selected immature female rats, control lighting in 500 ~ 1900 hours, food and water at random. Induced ovulation and luteal formation: Immature female rats were injected with 8 IU / 0.2 ml of progesterone (PMSG) 8 IU / 0.2 ml saline on the 28th morning to promote follicular development, and endogenous LH / FSH was released two days later Ovulation. Isolation and Incubation of Lutein: Rats were sacrificed on days 31, 33 and 35, ovaries removed rapidly, and lutealized by hand under a stereomicroscope and set at l, 3,5 days respectively. The incubation solution was a modified Krebs Ringer bicarbonate buffer, pre-incubated at 37 ° C