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绞股蓝外植体在不同激素浓度的MS培养基上培养,经过正交试验选择了四种最适培养基配方。形态观察和同工酶测定结果表明,在MS基本培养基中添加NAA(1.0～2.0mg/L)可促进愈伤组织和根的生长;添加6-BA(2.0mg/L)也能诱导愈伤组织,并能分化丛生芽;而在6-BA(1.0mg/L)中加入低浓度的NAA(0.1mg/L)和2,4-D(0.1mg/L),对诱导愈伤组织和丛生芽的分化有增效作用。经染色体观察和试管苗大田试种,未发现遗传性变异。
Gynostemma explants were cultured on MS medium with different hormone concentrations. Four kinds of optimal medium formulas were selected through orthogonal test. Morphological observation and isozyme assay showed that the addition of NAA (1.0-2.0 mg / L) to MS medium could promote the growth of callus and root. The addition of 6-BA (2.0 mg / L) also induced But also could differentiate into clusters of buds. However, low concentrations of NAA (0.1 mg / L) and 2,4-D (0.1 mg / L) were added to 6-BA Differentiation and clustered bud synergistic effect. Chromosome observation and in vitro seedling field trial, found no genetic variation.