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目的:建立高效液相色谱/串联质谱法测定肿节风分散片中异嗪皮啶。方法:采用Agilent SB-C18色谱柱(2.1 mm×50 mm,1.8μm),流动相甲醇-水(35∶65),流速0.2 mL·min-1。三重四级杆质谱检测器,正离子多反应监测模式(MRM),监测离子(m/z)222.9/189.7,222.9/161.9。结果:异嗪皮啶在0.48~2.40 mg·L-1与峰面积具有良好的线性关系(r=0.999 2),平均加样回收率为99.3%,RSD 0.55%(n=9)。结论:方法简便易行,结果准确,重复性好,可用于肿节风分散片中异嗪皮啶的含量测定。
Objective: To establish a HPLC / tandem mass spectrometry method for the determination of isopractin in swollenang dispersible tablets. Methods: The mobile phase was methanol-water (35:65) with a flow rate of 0.2 mL · min-1 using an Agilent SB-C18 column (2.1 mm × 50 mm, 1.8 μm) Triple quadrupole mass spectrometry detector, positive ion multiple reaction monitoring mode (MRM), monitored ions (m / z) 222.9 / 189.7, 222.9 / 161.9. The results showed that isopinarubine had a good linear relationship (r = 0.999 2) with the peak area of 0.48-2.40 mg · L-1 and the average recovery was 99.3% and the RSD was 0.55% (n = 9). Conclusion: The method is simple and easy to operate with accurate results and good reproducibility. It can be used for the determination of isopractin in swollen-air dispersible tablets.