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在 36只麻醉Sprague Dawley大鼠 ,观察了最后区内微量注射辣椒素 (10 μmol/L ,5 0nl)对平均动脉压(MAP)、心率 (HR)和肾交感神经放电 (RSNA)的影响。实验结果如下 :(1)最后区内注射辣椒素可引起MAP、HR和RSNA明显增加 ,分别由 12 34± 0 5 3kPa、32 8 5 2± 7 5 4bpm和 10 0± 0 %增至 15 17± 0 2 5kPa (P <0 0 0 1)、35 4 81± 8 5 4bpm (P <0 0 0 1)和 15 6 95± 7 5 7% (P <0 0 0 1) ;(2 )静脉注射辣椒素受体阻断剂钌红 (10 0mmol/L ,0 2ml)后 ,辣椒素的上述效应可被明显抑制 ;(3)预先应用NMDA受体阻断剂MK 80 1(5 0 0 μg/kg,0 2ml,iv)也明显抑制辣椒素的兴奋效应。以上结果提示 ,最后区微量注射辣椒素对血压、心率和肾交感神经放电有兴奋作用 ,而此作用由辣椒素受体介导并有谷氨酸参与
In 36 anesthetized Sprague Dawley rats, the effect of capsaicin (10 μmol / L, 500 nl) on mean arterial pressure (MAP), heart rate (HR) and renal sympathetic nerve discharge (RSNA) The experimental results are as follows: (1) The last regional injection of capsaicin caused a significant increase in MAP, HR and RSNA, respectively, from 12 34 ± 0 5 3 kPa, 32 8 5 2 ± 7 5 4bpm and 10 0 ± 0% to 15 17 (P <0.01), 35 4 81 ± 8 5 4bpm (P 0 01) and 15 6 95 ± 7 57% (P 0 01 0) The above effect of capsaicin can be significantly inhibited by the injection of capsaicin receptor blocker ruthenium red (10 0 mmol / L, 0 2 ml); (3) pretreatment with NMDA receptor blocker MK 80 1 (500 μg / kg, 0 2ml, iv) also significantly inhibited the excitatory effect of capsaicin. The above results suggest that microinjection of capsaicin into the final zone is stimulated by blood pressure, heart rate and renal sympathetic nerve discharge, whereas this effect is mediated by capsaicin receptor with glutamate involvement