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目的 了解输尿管移行细胞癌组织中bcl 2和TGF β1 蛋白的表达。方法 采用免疫组化技术 ,检测 5 2例输尿管移行细胞癌与 1 1例正常输尿管移行上皮细胞中bcl 2和TGF β1 蛋白的表达。结果 1 1例正常输尿管中bcl 2蛋白表达阳性 2例 (1 8.2 % ) ,TGF β1 蛋白表达阳性 1 1例 (1 0 0 % )。 5 2例输尿管移行细胞癌中bcl 2蛋白表达阳性 2 0例 (3 8.5 % ) ,TGF β1 蛋白表达阳性 2 6例 (5 0 % )。bcl 2阳性率在高、中、低分化癌中分别为 2 1 .1 %、3 6 .4%和 72 .7%。在TiS～T1 和T2 ～T4期阳性率分别为 3 1 .6 %和5 7.1 %。TGF β1 阳性率在高、中、低分化癌中分别为 73 .7%、45 .5 %和 1 8.2 %。在TiS～T1 和T2 ～T4期阳性率分别为 6 0 .5 %和 2 1 .4%。在输尿管移行细胞癌中bcl 2表达程度随肿瘤分级增加而增加 ,在各级肿瘤分化间差异有显著性 (χ2 =7.93 ,P <0 .0 5 )。浅表性癌bcl 2表达阳性率低于浸润性癌 ,但差异无显著性 (χ2 =2 .82 ,P >0 .0 5 )。随肿瘤分级增加 ,TGF β1 阳性率下降 (χ2 =8.90 ,P <0 .0 5 ) ,随肿瘤由浅表型向浸润型转化 ,阳性率下降 (χ2 =6 .2 6 ,P <0 .0 5 ) ,差异均有显著性。结论 bcl 2和TGF β1 参与了输尿管移行细胞癌的发生发展过程 ,可作为评估其生物行为的瘤标。
Objective To understand the expression of bcl 2 and TGF β 1 protein in transitional cell carcinoma of the ureter. Methods Immunohistochemistry was used to detect the expression of bcl 2 and TGF β 1 protein in 52 patients with ureteral transitional cell carcinoma and 11 normal ureteral transitional epithelial cells. Results The expression of bcl 2 protein was positive in 1 case of normal ureter in 2 cases (1 8.2%), and the expression of TGF β 1 protein was positive in 11 cases (100%). In 52 cases of transitional cell carcinoma of the ureter, bcl-2 protein expression was positive in 20 cases (38.5%), and TGF-beta1 protein expression was positive in 26 cases (50%). The positive rate of bcl 2 was 21.1%, 36.4%, and 72.7% in high, medium, and low differentiated cancers, respectively. The positive rates in TiS~T1 and T2~T4 were 31.6% and 57.1%, respectively. The positive rate of TGFβ1 was 73.7%, 45.5%, and 18.2% in high, middle, and low differentiated cancers, respectively. The positive rates in TiS-T1 and T2-T4 were 60.5% and 21.4%, respectively. The degree of bcl-2 expression in ureteral transitional cell carcinoma increased with the grade of tumor, and there was a significant difference in tumor differentiation at various levels (χ2 = 7.93, P < 0.05). The positive rate of bcl-2 expression in superficial carcinoma was lower than that in invasive carcinoma, but the difference was not significant (χ2 = 2.82, P > 0.05). With the increase of tumor grade, the positive rate of TGFβ1 decreased (χ2 = 8.90, P < 0.05), and the rate of positive change decreased with tumor progression from superficial to infiltrating (χ2 = 6.26, P < 0.05). ), the differences are significant. Conclusion bcl 2 and TGF β 1 are involved in the development of ureteral transitional cell carcinoma and can be used as a tumor marker to evaluate their biological behavior.