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目的:研究葛根素对帕金森病细胞模型的保护作用及其具体的作用机理。方法:用0.16mM的MPP+处理PC12细胞48h建立帕金森病细胞模型。实验分为对照组、损伤组和保护组,损伤组用MPP+(0.16mM)处理PC12细胞;保护组用葛根素提前预处理PC12细胞1h,后加MPP+。检测PC12细胞存活率、Caspase-3活性及ERβ的转录活性。结果:葛根素能够抑制caspase-3的激活,且其依赖于ERβ的表达,雌激素受体拮抗剂ICI 182,780可阻断上述效应;其次葛根素可提高ERβ的转录活性。结论:葛根素对MPP+诱导损伤的PC12细胞具有抗细胞凋亡的保护作用,且具体的作用机理可能依赖于ERβ介导的经典的基因组作用模式。
Objective: To study the protective effect of puerarin on Parkinson’s disease cell model and its specific mechanism of action. Methods: PC12 cells were treated with 0.16mM MPP + for 48h to establish Parkinson’s disease cell model. The experimental group was divided into control group, injury group and protective group. PC12 cells were treated with MPP + (0.16mM) in injury group, and PC12 cells were pretreated with puerarin in protection group for 1h and MPP +. The survival rate of PC12 cells, Caspase-3 activity and ERβ transcriptional activity were detected. Results: Puerarin inhibited the activation of caspase-3, and its dependence on ERβ expression, estrogen receptor antagonist ICI 182,780 can block the above effects; followed by puerarin can increase ERβ transcriptional activity. CONCLUSION: Puerarin can protect PC12 cells induced by MPP + against apoptosis, and the specific mechanism of action may depend on the classical genomic pattern induced by ERβ.