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利用12对SSR引物,对来自云南元阳哈尼梯田不同海拔高度、不同水稻品种上的稻瘟病菌菌株进行遗传多样性分析,并运用日本清泽建立的鉴别品种对其中62个菌株进行生理小种鉴定。结果显示:稻瘟病菌总体遗传多样性水平较高(He=0.57±0.27,I=1.17±0.60)。聚类分析表明,在80%相似水平下菌株可划分为9个多样性群体。供试菌株划分为16个生理小种,其中002、000和006为优势小种。分离的不同海拔稻瘟病菌生理小种和遗传多样性存在差异。海拔1600～1700m生理小种最丰富,达9个生理小种;海拔1500～1600m、1400～1500m和1700～1800m,生理小种分别为6、5和4个;遗传多样性分析表明,从海拔1400～1500m分离的菌株遗传多样性水平最高(He=0.57±0.24),并划分为6个群体。综合分析表明,在元阳哈尼梯田系统中,栽培传统品种遗传多样性越丰富的区域,其稻瘟病菌群体多样性和生理小种也越丰富。
12 pairs of SSR primers were used to analyze the genetic diversity of M. grisea isolates from different rice varieties at different altitudes in Hani terraces in Yuanyang, Yunnan province. 62 strains of M. grisea Identification. The results showed that the overall level of genetic diversity of Magnaporthe grisea was high (He = 0.57 ± 0.27, I = 1.17 ± 0.60). Cluster analysis showed that the strains could be divided into 9 diversity groups at 80% similarity level. The test strains were divided into 16 physiological races, of which 002,000 and 006 were the dominant races. There were differences in physiological races and genetic diversity of Magnaporthe grisea isolated from different altitudes. The races with 1600 ~ 1700 m above sea level were the most abundant, with 9 physiological races at altitudes of 1500-1600 m, 1400-1500 m and 1700-1800 m, and physiological races of 6, 5 and 4, respectively. Genetic diversity analysis showed that from the altitude The strains isolated from 1400-1500 m had the highest genetic diversity (He = 0.57 ± 0.24) and were divided into 6 groups. Comprehensive analysis showed that in Yuanyang Hani terraced fields, the areas with more genetic diversity of traditional cultivars were more rich in M. grisea population and race.