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本实验用白色雌性大耳家免21只,由静脉注入大肠杆菌内毒素复制发热模型,实验组给内毒素20分钟后在动物的百会穴施以电针,给内毒素后80分钟(动物发热的高峰期)分别从动物的小脑延髓池抽取脑脊液,由耳缘静脉采血、用蛋白结合法测定脑脊液和血浆中cAMP含量。给实验组动物施以电针后体温上升受到明显的抑制。脑脊液中cAMP含量较对照组动物明显减少,但血浆中cAMP含量差异不显著。电针抑制体温上升的作用,很可能是抑制了脑组织产生和释放cAMP有关,而与血浆中cAMP含量无关。本文也进一步证实了cAMP是重要的中枢性发热介质。
In this experiment, 21 white females were used to exempt the 21st big-eared family and the endotoxin was replicated by intravenous injection of E. coli. The experimental group was given electro-acupuncture at the Baihui acupoint of the animal 20 minutes after the endotoxin was given, and 80 minutes after the endotoxin administration (animal In the peak period of fever, cerebrospinal fluid was collected from the cerebellomedullary cistern of the animals, blood was collected from the marginal ear vein, and the cAMP content in cerebrospinal fluid and plasma was determined by protein binding method. The increase in body temperature after the electroacupuncture was applied to the experimental animals was significantly inhibited. The cAMP content in cerebrospinal fluid was significantly reduced compared with the control animals, but the plasma cAMP content was not significantly different. The effect of electroacupuncture in inhibiting the increase of body temperature is likely to be related to inhibition of the production and release of cAMP in brain tissue, but not to the cAMP content in plasma. This article also further confirmed that cAMP is an important central heating medium.