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沼气中的硫化氢(H2S)是一种具有腐蚀性的有毒气体,因此在使用前必须对沼气进行人工脱硫处理.以硝酸盐作为电子受体,设置两个反应器研究在不同沼气进气速率下微生物对H2S去除能力的影响,并分析厌氧污泥的菌群结构和关键微生物种群类型.结果表明在接种污泥的反应器A中,在低进气H2S负荷下,即沼气流速为0.5 L/min时,H2S的去除率在96%以上;在高进气H2S负荷下,即沼气流速为2 L/min、3 L/min和4 L/min时,H2S去除率在20%-35%之间.即随着沼气流速加快,气体停留时间缩短,H2S去除率逐渐下降.未接种污泥的反应器B前6 d H2S去除率逐渐升高,6 d后去除率逐渐下降.厌氧污泥中的微生物生化作用对H2S的去除率有显著影响.微生物群落结构分析结果表明,在反应器运行过程中污泥的微生物种类变化较小.厌氧污泥中微生物群落的优势门是变形菌门(Proteobacteria),优势属是硫杆菌属(Thiobacillus).Sulfurimonas在微生物群落中的相对丰度变化与H2S去除率的变化呈显著正相关.故Sulfurimonas可能是本反应系统中的主要脱氮脱硫菌.
Hydrogen sulfide (H2S) in biogas is a kind of corrosive toxic gas, so biogas must be artificially desulfurized before use.With nitrates as electron acceptor, two reactors were set up to study the effect of biogas on the biogas inlet rate The effect of microorganisms on the removal of H2S was analyzed and the anaerobic sludge flora structure and key microbial population types were analyzed.The results showed that under the low inlet H2S load in the reactor A inoculated with sludge, the biogas flow rate was 0.5 The removal rate of H2S is above 96% at L / min and under the condition of high inlet H2S load, ie, 2 L / min, 3 L / min and 4 L / min, the removal rate of H2S is between 20% -35 % .With the accelerated biogas flow rate, gas residence time shortened, H2S removal rate decreased.Under the sludge inoculation reactor B H2S removal efficiency gradually increased 6 d, 6 d after the removal rate decreased gradually.Animal The biochemical effect of microorganisms in sludge has a significant impact on the removal rate of H2S.The results of microbial community structure analysis show that the change of microbial species in sludge is small during the operation of reactor.The advantage of microbial community in anaerobic sludge is deformation Proteobacteria, the dominant genus is Thiobacillus (Thiobacillu s). The relative abundance of Sulfurimonas in microbial communities was positively correlated with the change of H2S removal rate, so Sulfurimonas may be the main denitrifying bacteria in this reaction system.