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目的探索黄芪改善注射性坐骨神经损伤大鼠肌萎缩的机制。方法 SD大鼠24只,♀♂不拘,随机分为4组:正常组、模型组、阴性对照组和黄芪干预组。采用天平称量肌重,HE染色后Leica图像分析仪测量肌纤维横切面积,VG染色法观察腓骨长肌内胶原纤维的变化。结果青霉素注射坐骨神经后2周,与正常组相比,模型组大鼠跛行,腓骨长肌肌块缩小,色泽变暗,肌重减轻,肌纤维横切面积减小,胶原纤维明显增生;阴性对照组进一步加重;黄芪干预组上述变化明显好转。结论黄芪能通过减少大鼠腓骨长肌胶原纤维增生,改善青霉素注射坐骨神经损伤引起的肌萎缩。
Objective To explore the mechanism of Astragalus membranaceus in improving amyotrophy in rats with injected sciatic nerve injury. Methods Twenty-four SD rats were randomly divided into 4 groups: normal group, model group, negative control group and Astragalus intervention group. Weighing the balance using a balance, HE staining Leica image analyzer measured muscle fiber cross-sectional area, VG staining observed fibula long muscle collagen fibers. Results Compared with the normal group, the rats in the model group became limp, the muscle of the long fibula became smaller, the color became darker, the weight of the muscle decreased, the cross-sectional area of the muscle fibers decreased and the collagen fibers proliferated obviously. The negative control group Further aggravate; Astragalus intervention group significantly improved the above changes. Conclusion Radix Astragali can improve muscle atrophy caused by sciatic nerve injury caused by injection of penicillin by reducing collagen hyperplasia in rat peroneus fibulae.