论文部分内容阅读
目的 主要对99mTc MIBISPECT脑胶质瘤显像与病理分级及肿瘤细胞核增殖抗原 (PCNA)之间的关系进行临床研究。方法 对 16例脑胶质瘤患者行99mTc MIBISPECT早期相和延迟脑断层显像。对早期相和延迟相显像分别作半定量分析。以得99mTc MIBI早期、延迟相摄取指数和滞留指数。所有患者手术切除标本切片行常规苏木精 伊红染色 ,并对切片行核增殖抗原 (PCNA)单克隆抗体免疫组化染色 ,以了解肿瘤细胞增殖状况。结果 病理诊断星型胶质细胞瘤 6例 ,间变性星型胶质细胞瘤 5例 ,多形性胶质母细胞瘤 5例。星型胶质细胞瘤、间变性星型胶质细胞瘤和多形性胶质母细胞瘤早期和延迟相99mTc MIBI摄取指数和滞留指数分别为 1.0 7± 0 .0 8,1.12± 0 .35 ,1.0 4± 0 .0 8;1.5 9± 0 .2 8,1.78± 0 .2 1,1.12± 0 .12 ;4.98± 1.2 5 ,7.5 3± 1.39,1.5 3± 0 .19。经统计学检验 ,星型胶质细胞瘤与多形性胶质母细胞瘤、间变性胶质细胞瘤和多形性胶质母细胞瘤的延迟相99mTc MIBI摄取指数和滞瘤指数差异均有显著性 ,P均 <0 .0 1。另对各组胶质细胞瘤的PCNA评分与99mTc MIBI滞留指数的相关系数进行计算 ,结果r =0 .747,P <0 .0 1。结论 99mTc MIBI脑胶质瘤SPECT可对术前肿瘤分级提供一定帮助。滞留指数可对肿瘤细胞的增殖活力?
Objective To investigate the relationship between 99mTc MIBISPECT glioma imaging and pathological grading and tumor cell nuclear antigen (PCNA). Methods 16 cases of glioma patients underwent 99mTc MIBISPECT early phase and delayed brain imaging. Semi-quantitative analysis of early phase and delayed phase imaging respectively. In order to get 99mTc MIBI early, delayed phase uptake index and retention index. All patients undergone surgical resection of the specimen were stained with hematoxylin and eosin. Immunohistochemical staining of monoclonal antibody against nuclear proliferative antigen (PCNA) was performed on the sections to observe the proliferation of tumor cells. Results Pathological diagnosis of astrocytoma in 6 cases, anaplastic astrocytoma in 5 cases, 5 cases of glioblastoma multiforme. Astrocytoma, anaplastic astrocytoma and glioblastoma early and delayed phase 99mTc MIBI uptake index and retention index were 1.07 ± 0 .08, 1.12 ± 0.35 , 1.0 4 ± 0.08; 1.5 9 ± 0.28, 1.78 ± 0.2, 1.12 ± 0.12; 4.98 ± 1.2 5, 7.5 3 ± 1.39, 1.5 3 ± 0.19. Statistically, there was a significant difference between the uptake of 99mTc MIBI and the hysterophageal index of astrocytoma and glioblastoma multiforme, anaplastic glioblastoma and glioblastoma multiforme Significance, P <0.01. In addition, the correlation coefficient of PCNA score and retention index of 99mTc MIBI in each group was calculated. The result was r = 0.747, P <0.01. Conclusion 99mTc MIBI glioma SPECT may be helpful in the classification of preoperative tumors. Detention index of tumor cell proliferation activity?