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本文作者从猪蛔虫中分离出编码过氧化氢酶(AsCAT)的cDNA全序列并对其基本结构进行了分析。 作者从屠宰场收集猪蛔虫成虫,分离生殖组织,用玻璃匀浆器将其制成匀浆,然后按常规方法分离DNA和RNA。设计CAT1S和CAT2AS两个简并引物,用猪蛔虫λgtllcDNA文库或猪蛔虫基因组DNA做PCR扩增。AsCAT cDNA和基因组DNA片段用琼脂糖凝胶电泳分析扩增产物,纯化后植入PCRⅡ质粒载体,以标准法测定核苷酸序列。
The authors isolated the cDNA sequence encoding catalase (AsCAT) from Ascaris suum and analyzed its basic structure. The authors collected Ascaris suum adult from abattoirs, isolated the reproductive tissue, homogenized it with a glass homogenizer and then isolated DNA and RNA by conventional methods. Two degenerate primers, CAT1S and CAT2AS, were designed and PCR amplification was performed using Ascaris suum λgtllcDNA or Ascaris suum genomic DNA. The AsCAT cDNA and genomic DNA fragments were analyzed by agarose gel electrophoresis of the amplified products, which were then inserted into the PCR II plasmid vector and the nucleotide sequence was determined by standard methods.