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目的以大鼠组织匀浆及ＲＢＣ为材料研究３种穿心草口山酮（ｘａｎｔｈｏｎｅ，Ｘａｎ）：１，８－ｄｉｈｙｄｒｏｘｙ３，５－ｄｉｍｅｔｈｏｘｙｘ－ａｎｔｈｏｎｅ（Ｘａｎ－Ⅰ）；１－ｈｙｄｒｏｘｙ，５－ｄｉｍｅｔｈｏｘｙｘａｎｔｈｏｎｅ（Ｘａｎ－Ⅱ）；１－ｈｙｄｒｏｘｙ，３，７，８－ｔｒｉｔｈｏｘｙｘａｎｔｈｏｎｅ（Ｘａｎ－Ⅲ）抗脂质过氧化作用。方法以ＭＤＡ为指标，观察Ｘａｎ对组织匀浆体外脂质过氧化及ＲＢＣ自氧化溶血体系脂质过氧化产物丙二醛（ＭＤＡ）生成量的影响；并观察Ｘａｎ对邻苯三酚自氧化产生的Ｏ·２及Ｃｕ２＋－ＶｉｔＣ自由基产生系统产生的·ＯＨ的清除作用。结果①０．０５μｍｏｌ·Ｌ－１的３种Ｘａｎ均可抑制正常大鼠脑、肝、心匀浆体外过氧化脂质生成，并能对抗半胱氨酸和硫酸亚铁所致过氧化脂质生成增加。②３种Ｘａｎ均能抑制ＲＢＣ自氧化，显著减少ＲＢＣ自氧化过程中ＭＤＡ的含量。③３种Ｘａｎ均能清除超氧阴离子自由基（Ｏ·２）和羟自由基（·ＯＨ），清除·ＯＨ的作用更强。④３种Ｘａｎ清除氧自由基的活性强弱顺序为Ｘａｎ－Ⅰ＞Ｘａｎ－Ⅲ＞Ｘａｎ－Ⅱ。结论穿心草口山酮具有一定的抗氧化作用，能直接清除活性氧自由基。
Objective To study three kinds of xanthone (Xan) in rat homogenate and RBC: 1,8-dihydroxy3,5-dimethoxyx-anthone (Xan-I); 1-hydroxy, 5-dimethoxyxanthone (Xan-II); 1-hydroxy, 3,7,8-trithoxyxanthone (Xan-III) anti-lipid peroxidation. Methods The effect of Xan on the lipid peroxidation of tissue homogenate and the production of malondialdehyde (MDA) of lipid peroxidation product in hemolysis system of RBC were observed with MDA as the index. The effect of Xan on the autooxidation of pyrogallol was observed. O·2 and Cu2+-VitC radical generating system · OH scavenging effect. Results Three kinds of Xan (10.05μmol·L-1) inhibited the production of lipid peroxides in brain, liver and heart homogenate of normal rats, and were able to inhibit the production of lipid peroxidation caused by cysteine and ferrous sulfate. increase. All of the 23 kinds of Xan could inhibit the auto-oxidation of RBC and significantly reduce the content of MDA in the course of auto-oxidation of RBC. 33 kinds of Xan can remove superoxide anion radical (O·2) and hydroxyl radical (·OH), and the effect of eliminating OH is stronger. The order of the activity of the 43 Xan scavenging oxygen free radicals was Xan-I>Xan-III>Xan-II. Conclusion The xanthone from Tongxincao has certain antioxidation effect and can directly scavenge active oxygen free radicals.