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采用花粉管通道法将甜菜碱醛脱氢酶基因(BADH)转入玉米自交系京24,以提高玉米的耐盐性。在玉米植株T0代喷洒200mg/L草铵膦除草剂进行抗性试验,获得4株抗性苗。在T0代进行PCR初步检测得到3株转基因植株。在T2代通过Southern blotting以及Northern blotting检测,表明外源BADH基因已经整合并表达。对T2代株系和京24自交系(CK)在高盐胁迫下进行相对含水量、叶片电解质外泄率、甜菜碱含量、脯氨酸含量等生理指标的测定,结果表明转基因株系的生理指标明显好于对照,说明转入BADH基因能提高玉米的耐盐性。
The betaine aldehyde dehydrogenase gene (BADH) was transferred into maize inbred line Jing-24 by using the pollen tube pathway to improve the salt tolerance of maize. T0 plants were sprayed with 200mg / L glufosinate ammonium herbicide resistance test to obtain four resistant seedlings. T0 generation in the initial PCR test obtained three transgenic plants. In T2 generation by Southern blotting and Northern blotting assay, the exogenous BADH gene has been integrated and expressed. The relative water content, leaf electrolyte leakage rate, betaine content and proline content in T2 generation and Beijing 24 inbred lines (CK) under high salt stress were measured. The results showed that the transgenic lines Physiological indicators were significantly better than the control, indicating that BADH gene transfer to improve the salt tolerance of corn.