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根据我国恶性疟原虫株MSP-2基因序列,检索我国虫株MSP-2是否具有国外已鉴定的MSP-2抗原表位。结果显示,我国虫株MSP-2具有已鉴定的FC-27等位基因型可变区抗原表位STNS和DTPTATE。同时应用计算机辅助抗原表位分析技术对MSP-2进行抗原表位分析预测,并以合成肽技术鉴定了预测表位的免疫原性。抗原指数分析表明,MSP-2分子亲水性指数和侧链易曲性指数最高的区域分别位于aa153～166和aa65～72,而aa53～60可能是我国虫株特异的抗原表位。用4个合成肽进行免疫学鉴定,证实预测的表位可以诱导抗肽抗体反应,免疫血清可识别重组MSP-2和恶性疟原虫全抗原中MSP-2抗原区带,且合成肽可与我国流行区高度免疫人群免疫血清反应。由于这些表位存在于我国海南、云南、安徽株MSP-2序列中,可以作为研制我国恶性疟重组多价表位疫苗的候选抗原表位。
According to the sequence of MSP-2 gene of Plasmodium falciparum in our country, we searched whether the MSP-2 strain in our country has the MSP-2 epitope that has been identified abroad. The results showed that our strain MSP-2 has identified the FC-27 allelic variant epitopes STNS and DTPTATE. At the same time, the epitope of MSP-2 was predicted by computer-assisted epitope analysis and the immunogenicity of the predicted epitopes was identified by the synthetic peptide technique. Antigen index analysis showed that the region with the highest hydrophilic index and the highest susceptibility index of MSP-2 was located in aa153-166 and aa65-72, respectively, while aa53-60 was probably a pest-specific epitope in China. Immunoassay with four synthetic peptides confirmed that the predicted epitopes could induce an anti-peptide antibody response. The immune sera recognized the MSP-2 antigenic region in recombinant MSP-2 and Plasmodium falciparum whole antigens, and the synthetic peptides were compatible with our country Epidemic area highly immunized people immune serum reaction. Because these epitopes exist in the MSP-2 sequences of Hainan, Yunnan and Anhui strains in China, they can be used as candidate epitopes for the development of the recombinant multivalent epitope vaccine against P. falciparum in China.