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目的探讨木脂素对醋酸铅染毒小鼠生精功能的影响及其作用机制。方法将昆明种雄性小鼠60只随机分为对照组、模型组、阳性对照组、木脂素组,除对照组外,其余3组小鼠均腹腔注射醋酸铅(40 mg/kg),连续10 d,建立雄性小鼠生精障碍模型;造模后第2 d开始,阳性对照组小鼠腹腔注射绒毛膜促性腺激素(500 IU/kg),木脂素组小鼠给予木脂素(200 mg/kg)灌胃,对照组及模型组小鼠灌胃等量生理盐水,连续30 d;末次给药24h后,处死小鼠,取睾丸和附睾,检测睾丸组织超氧化物歧化酶(SOD)、乳酸脱氢酶(LDH)、三磷酸腺苷(ATP)酶含量。比较各组小鼠精子存活率。苏木精-伊红染色观察睾丸组织形态。结果与对照组比较,模型组小鼠精子活率[(53.2±1.3)%]降低,LDH、SOD、Mg2+、Ca2+-ATP酶活性[分别为(0.18±0.03)U/gprot、(16.80±2.23)U/mgprot、(31.03±4.87)、(8.12±1.24)μmol Pi/(mgprot·hour)]降低,差异有统计学意义(P<0.01);与模型组比较,木脂素组小鼠精子活率[(68.7±2.2)%]升高;LDH、SOD、Mg2+、Ca2+-ATP酶活性[分别为(0.23±0.09)U/gprot、(23.01±3.86)U/mgprot、(64.21±10.49)、(17.20±5.18)μmol Pi/(mgprot·hour)]升高,差异有统计学意义(P<0.05)。结论木脂素对醋酸铅所致生精障碍小鼠的生殖功能具有一定保护作用,其机制可能与抗氧化损伤有关。
Objective To investigate the effect of lignan on the spermatogenesis in mice induced by lead acetate and its mechanism. Methods Sixty Kunming male mice were randomly divided into control group, model group, positive control group and lignan group, except the control group, the other three groups were injected with lead acetate (40 mg / kg) d, the establishment of male mice model of spermatogenesis; starting 2d after modeling, the positive control group mice intraperitoneal injection of human chorionic gonadotropin (500IU / kg), lignans group of mice given lignan (200 The rats in the control group and model group were given the same amount of normal saline for 30 days. After the last administration, the mice were sacrificed and the testis and epididymis were taken. The activities of superoxide dismutase (SOD) ), Lactate dehydrogenase (LDH), adenosine triphosphate (ATP) enzyme content. The sperm survival rate of mice in each group was compared. Hematoxylin-eosin staining testicular tissue morphology. Results Compared with the control group, the sperm motility in the model group was significantly lower than that in the control group [(53.2 ± 1.3)%] (0.18 ± 0.03 U / gprot, (16.80 ± 2.23 (P <0.01). Compared with the model group, the lignan-treated mouse sperm (P <0.01) showed no significant difference The activity of LDH, SOD, Mg2 + and Ca2 + -ATP were significantly higher than that of the control group ([(68.2 ± 2.2)%] , (17.20 ± 5.18) μmol Pi / (mgprot · hour)], the difference was statistically significant (P <0.05). Conclusion Lignans have a protective effect on reproductive function in mice induced by lead acetate. The mechanism may be related to the anti-oxidative damage.