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报道了HPLC法测定血清中茶碱浓度。血清用量只需100μl。采用了甲醇:水(1:2)为流动相,以喘定作为内标物。常用配伍药无干扰。回收率约95%,变异系数小于5%。
Reported the HPLC method for the determination of serum theophylline concentration. Serum dosage only 100μl. Methanol: water (1: 2) was used as mobile phase and asthma was used as internal standard. Common compatibility drugs without interference. Recovery rate of about 95%, coefficient of variation of less than 5%.