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目的和方法：应用心脏微血管内皮细胞体外培养模型，研究不同浓度的ＴＮＦα、ＩＬ－１β、ＩＬ－６对微血管内皮与中性粒细胞粘附的调节。结果：５×１０４Ｕ／Ｌ的ＴＮＦα和ＩＬ－１β可明显增加内皮细胞与中性粒细胞的粘附（诱导内皮６ｈ），并分别于１０５Ｕ／Ｌ、２×１０５Ｕ／Ｌ浓度时使中性粒细胞粘附数达高峰，但不同浓度的ＩＬ－６均未使粘附增加，免疫组化ＡＢＣ法证实了同样的结果。进一步用抗ＩＣＡＭ－１和抗ＬＦＡ－１单克隆抗体阻断实验表明，两种抗体在５ｍｇ／Ｌ浓度下可显著阻断中性粒细胞与内皮细胞间的粘附。结论：心脏微血管内皮细胞与中性粒细胞的粘附主要通过粘附分子ＩＣＡＭ－１／ＬＦＡ－１介导。
PURPOSE AND METHODS: The effects of different concentrations of TNFα, IL-1β and IL-6 on the adhesion of microvascular endothelial cells to neutrophils were studied in vitro using cardiac microvascular endothelial cells. Results: TNFα and IL-1β of 5 × 10 4 U / L significantly increased the adhesion of endothelial cells to neutrophils (induced endothelium for 6 h), and at the concentrations of 105 U / L and 2 × 105 U / L, Cell adhesion reached the peak, but different concentrations of IL-6 did not increase adhesion, immunohistochemistry ABC method confirmed the same results. Further blocking experiments with anti-ICAM-1 and anti-LFA-1 monoclonal antibodies showed that both antibodies significantly blocked neutrophil-endothelial cell adhesion at a concentration of 5 mg / L. Conclusion: The adhesion of cardiac microvascular endothelial cells to neutrophils is mainly mediated by the adhesion molecule ICAM-1 / LFA-1.