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目的探讨结节型淋巴细胞为主型霍奇金病临床病理特征，方法结节型淋巴细胞为主型霍奇余病淋巴结石蜡包埋组织11例，冰冻组织1例。除HE切片外，还用抗CD3，CD19，CD20，CD30，CD45RB;及κ、λ中克隆抗体进行ABC法免疫组织化学标记，1例冰冻组织采用从组织切片上提取单个细胞行聚合酶链反应（PCR）。结果肿瘤组织以结节状排列的小淋巴细胞和组织细胞为背景，其间散在分布L＆H细胞。L＆H细胞表达CD19（10例），CD20（12例），CD45RB（12例）及κ轻链（11例），1例冰冻组织的单个L＆H细胞出现lgH和Vκ4家庭基因的重排。8例患者随访期超过5年以上。结论结节型淋巴细胞为主型霍奇金病临床过程非常缓慢，预后良好，其实质是一种特殊的B细胞肿瘤。
Objective To investigate the clinicopathological features of nodular lymphocyte-predominant Hodgkin’s disease. Methods The paraffin-embedded tissues of nodular lymphocyte-predominant Hodgkin’s disease were 11 cases of paraffin-embedded tissue and 1 case of frozen tissue. In addition to HE slices, anti-CD3, CD19, CD20, CD30, CD45RB; and kappa, lambda antibodies were used for ABC immunohistochemical labeling, and one frozen tissue sample was extracted from tissue sections by polymerase chain reaction. (PCR). RESULTS: The tumor tissues consisted of nodule-like small lymphocytes and histiocytes, with scattered L&H cells. L&H cells expressed CD19 (10 cases), CD20 (12 cases), CD45RB (12 cases), and kappa light chain (11 cases). A single L&H cell of frozen tissue showed rearrangement of lgH and VK4 family genes. Eight patients were followed up for more than 5 years. Conclusion The clinical course of nodular lymphocyte-predominant Hodgkin’s disease is very slow and the prognosis is good. The substance is a special type of B-cell tumor.