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目的 :研究抗人血管内皮生长因子 (VEGF)单抗E1 1 抑制颊癌组织的形态学改变 ,并探讨E1 1 抑癌效应的机制。方法 :接种人颊鳞癌的BALB c裸小鼠分别通过腹腔或瘤周皮下注射抗人VEGF单抗或生理盐水。接种后第 18天处死 ,取颊癌组织进行光镜及透射电镜观察。结果 :E1 1 组颊癌组织中血管分布稀少、存在囊性变坏死区及癌细胞坏死、凋亡等。此外还有血管内皮细胞变性、凋亡及血管壁受破坏、管腔变窄和堵塞等血管系统的异常改变。而生理盐水组癌组织血管丰富 ,颊癌组织结构完整。结论 :抗人VEGF单抗是通过抑制颊癌的血管生成而产生抑癌效应
OBJECTIVE: To study the morphological changes of cheek cancer tissues induced by anti-human vascular endothelial growth factor (VEGF) monoclonal antibody E1 1 and to explore the mechanism of E1 1 tumor suppressor effect. Methods: BALB / c mice injected with human cheek squamous cell carcinoma were injected subcutaneously with peritoneal or peritumoral anti-human VEGF monoclonal antibody or saline respectively. On the eighteenth day after inoculation, the mice were sacrificed, and the buccal carcinoma tissues were observed under light microscope and transmission electron microscope. Results: There was scarce vascular distribution in the buccal carcinoma of E1 group 1, cystic necrosis and necrosis and apoptosis of cancer cells. In addition there are vascular endothelial cell degeneration, apoptosis and destruction of the vessel wall, lumen narrowing and blockages and other vascular abnormalities. However, the normal saline group had abundant blood vessels and cheek cancer tissues were intact. Conclusion: The anti-human VEGF monoclonal antibody inhibits the angiogenesis of buccal carcinoma to produce tumor suppressor effect