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U sing different m odels of focal cerebral ischemia,the temporal and spatial rules of m etabolism and energy changes in the post- ischem ia brain tissue were measured by proton m agnet- ic resonance spectroscopy(1 HMRS) to provide valuable inform ation for judging the prognosis of a- cute focal cerebral ischemia and carrying out effective therapy.Nine healthy Sprague- Dawly rats (both sexes) were randomly divided into two groups:The rats in the group A(n=4 ) were occlud- ed with self- thrombus for1h;The rats in the group B(n=5 ) were occluded with thread- em boli for1h.The 1 H MRS at30 ,4 0 ,5 0 ,6 0 min respectively was examined and the m etabolic changes of NAA,Cho and L ac in the regions of interest were sem iquantitatively analyzed. The spectrum intregral calculus area ratio of NAA,Cho,L ac to Pcr+Cr was setas the criterion.The values of NAA· Cho in the regions of interest were declined gradually within 1h after ischem ia, especially,the ratio of Cho/ (Pcr+Cr) ,NAA/ (Pcr+Cr) at6 0 m in had significant difference with that at5 0 min(P<0 .0 5 ) .The ratio of L ac/ (Pcr+Cr) began to decrease at4 0 min from initial in- crease of L ac in both A and B groups.MR proton spectrum analysis was a non- invasive,directand com prehensive tool for the study of cellular metabolism and the status of the biochemical energy in acute ischem ia stroke.
U sing different m odels of focal cerebral ischemia, the temporal and spatial rules of m etabolism and energy changes in the post-ischem ia brain tissue were by proton m agnet-ic resonance spectroscopy (1 HMRS) to provide valuable inform for judging the prognosis of a-cute focal cerebral ischemia and carrying out effective therapy. Neine healthy Sprague-Dawly rats (both sexes) were randomly divided into two groups: The rats in the group A (n = 4) were occlud- ed with self- Thrombus forlh; The rats in the group B (n = 5) were occluded with thread-em boli for lh.The 1 H MRS at 30, 40, 50, 60 minutes respectively were examined and the etabolic changes of NAA, Cho and L ac in the regions of interest were sem iquantitatively analyzed. The spectrum of calculus area ratio of NAA, Cho, L ac to Pcr + Cr was setas the criterion. NA values of NAA · Cho in the regions of interest were subsequently countered 1h after ischem ia, especially, the ratio of Cho / (Pcr + Cr), NAA / (P Cr + Cr) at6 0 m in had significant difference with that at 50 min (P <0 .0 5). The ratio of L ac / (Pcr + Cr) began to decrease at 4 0 min from initial in- crease of L ac in both A and B groups. MR proton spectrum analysis was a non-invasive, direct and com prehensive tool for the study of cellular metabolism and the status of the biochemical energy in acute ischem ia stroke.