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目的:研究间隙连接蛋白家族中Cx26、Cx32、Cx43在前列腺癌(PCa)及良性前列腺增生(BPH)组织中的表达情况,分析三者与PCa生物学行为关系,探索其在PCa发生、发展中的作用机制,为PCa的诊断及治疗提供新的实验依据。方法:随机收集我院近4年存档的PCa石蜡标本31例、BPH 23例,采用免疫组化染色SABC法回顾性研究Cx26、Cx32、Cx43在PCa和BPH组织中的表达情况,半定量研究Cx26、Cx32、Cx43的表达与PCa、BPH的临床和病理参数的关系。结果:①Cx26、Cx32、Cx43在BPH、PCa组织中的阳性表达率分别为82.6%与74.2%(χ2=0.541,P>0.05)、78.3%与61.3%(χ2=1.763,P>0.05)和87.0%与38.7%(χ2=12.730,P<0.01),Cx43在PCa组织中的阳性表达率较BPH中显著降低。②Cx26、Cx43在PCa组织中的阳性表达强度与肿瘤的恶性程度呈负相关(r Cx26=-0.476,P<0.01;r Cx43=-0.484,P<0.01);Cx32的表达与肿瘤恶性程度无相关性(r=-0.242,P>0.05)。③3种Cx表达与年龄、血清PSA浓度及组织中PSA表达强度无相关性,且3种Cx间的表达亦无相关性。结论:Cx26、Cx32、Cx43在BPH和PCa组织中均有不同程度表达,其中Cx43在PCa的发生、发展中可能起到一定的作用,其也许可作为PCa除PSA外的另一标志物以及PCa生物治疗的新靶点,Cx26在PCa进展过程中可能起一定的作用,但其机制尚需进一步研究。
AIM: To investigate the expression of Cx26, Cx32 and Cx43 in prostate cancer (PCa) and benign prostatic hyperplasia (BPH) in the connexin family and to explore their relationship with the biological behavior of PCa and to explore their roles in the occurrence and development of PCa The mechanism of action for the diagnosis and treatment of PCa to provide a new experimental basis. Methods: 31 PCa paraffin specimens and 23 cases of BPH were collected randomly from our hospital in recent 4 years. The expression of Cx26, Cx32 and Cx43 in PCa and BPH tissues was retrospectively studied by immunohistochemical SABC method. Semiquantitative study of Cx26 , Cx32, Cx43 expression and PCa, BPH clinical and pathological parameters. Results ① The positive rates of Cx26, Cx32 and Cx43 in BPH and PCa tissues were 82.6% and 74.2% (χ2 = 0.541, P> 0.05), 78.3% and 61.3% (χ2 = 1.763, P> 0.05) % And 38.7% respectively (χ2 = 12.730, P <0.01). The positive expression rate of Cx43 in PCa tissues was significantly lower than that in BPH. The positive expression intensity of Cx26 and Cx43 in PCa tissues was negatively correlated with the malignancy degree (r Cx26 = -0.476, P <0.01; r Cx43 = -0.484, P <0.01). The expression of Cx32 was not correlated with the degree of malignancy (R = -0.242, P> 0.05). ③ There was no correlation between the three Cx expressions and the age, the serum PSA concentration and the PSA expression intensity in the tissues, and there was no correlation between the three Cx expressions. Conclusion: Cx26, Cx32 and Cx43 are expressed in different degrees in BPH and PCa tissues. Cx43 may play a role in the occurrence and development of PCa. It may be used as another marker of PCa in addition to PSA and PCa A new target of biotherapy, Cx26 may play a role in the progression of PCa, but its mechanism needs further study.