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水稻脆性突变体(嫩稻)是从γ射线诱变的籼稻品种双科早的M2代中筛选而来的茎、叶较脆的突变体,被命名为加fp1利用嫩稻和C-堡杂交的F2群体对fp1位点进行了精细的遗传定位.fp1位点首先被初步定位在水稻第3染色体着丝粒附近的微卫星 DNA分子标记 RM16和 STS分子标记 G144a之间,遗传距离分别为 3.1和 9.1cM.为了进一步定位fp1位点,在 RM16和 G144a间发展了一个CAPS分子标记 C524a,与fp1位点的遗传距离为 0.4 cM.这一结果为进一步构建覆盖fp1基因区域的 BAC重叠群和最终克隆fp1基因奠定了坚实的分子基础.等位性测定表明fp1与已知的水稻脆茎突变基因bc1 等位.
The rice brittle mutant (tender rice) is a mutant with more brittle stems and leaves, which was selected from the γ-ray induced indica rice variety Shuangkezao in the M2 generation. It was named plus fp1 by Nenjiang and C-Fort hybridization F2 population of fp1 locus for a fine genetic mapping. The fp1 locus was initially mapped between the microsatellite DNA marker RM16 and the STS marker G144a near the centromere of chromosome 3 in rice with genetic distances of 3.1 and 9.1 cM, respectively. In order to further locate the fp1 locus, a CAPS molecular marker C524a was developed between RM16 and G144a with a genetic distance of 0.4 cM from the fp1 locus. This result laid a solid molecular foundation for further construction of the BAC contig covering the fp1 gene region and the final cloned fp1 gene. An allelic assay indicated that fp1 is allelic to bc1 in the known rice crisp mutant gene.