ISOLATION,CHARACTERIZATION AND EXPRESSION OF THE COMPLEMENTARY DNA FOR HUMAN TUMOR NECROSIS FACTOR (

• 作 者: 肖蕾 田园 王秀琴 贾凤兰 吴旻
• 机 构: NationalLaboratoryofMolecularOncology,CancerInstitute,ChineseAcademyofMedicalSciences,Beijing100021,
• 出 处: Science in China,Ser.B
• 发表日期: 1990年10期
• 关键词: DNA mature plasmid template sequencing ligation monoclonal designated promoter E

A cDNA for human TNF-α (615bp) was isolated by means of polymerase chain reaction (PCR) using first strand cDNA from PMA-induced HL-60 cells as template. The result from sequencing the 615 bp cDNA fragment indicated that it corresponded to the entire sequ