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以甜柿为材料，对组培正常苗与玻璃苗的形态学差异进行了观察，研究了玻璃化现象发生的原因及防治方法。结果表明：培养体系的水分状 况不适是甜柿 玻璃苗发生 的主要原因。提高培养基中琼脂粉含量（ 达１０ ｇ／ Ｌ） 可降低培养基的水势，尤其是 降低了试管苗的水势，能有效地防止玻璃苗的发生，同时有利于继代苗的增殖和生长；适当减少每瓶中接种数目及缩短继代培养时间，也可降低玻璃苗的发生频率。
The persimmon was used as material to observe the morphological difference between normal tissue culture seedlings and glass seedlings and to study the cause of the vitrification and its prevention and cure methods. The results showed that the moisture condition of the culture system was the main reason for persimmon glass seedlings. Increasing agar powder content (up to 10 g / L) in the medium can reduce the water potential of the medium, especially reducing the water potential of the test tube seedlings, effectively preventing the occurrence of the glass seedling, and at the same time, it is favorable for the proliferation and growth of the seedling; Properly reduce the number of vaccination per bottle and shorten the subculture time, can also reduce the frequency of glass seedlings.