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本文研究了大豆花药培养中的培养基、基因型、激素配比、糖分种类及浓度、取材时期、预处理温度、接种方式、有机添加物等因素对愈伤组织诱导频率的影响。认为大豆花药在培养基上的脱分化启动具有群体效应，合适的取材时期是单核中晚期。高浓度蔗糖能抑制体细胞愈伤组织的产生，而愈伤组织的分化则需要较低的蔗糖浓度。愈伤组织在Ｂ＿５＋０．５ｍｇ／１ＮＡＡ＋１．０ｍｇ／１ＫＴ＋１％蔗糖和改良ＭＳ＋０．１ｍｇ／１ＩＢＡ＋０．１ｍｇ／１ＧＡ＿３＋０．４ｍｇ／１ＮＡＡ＋０．５ｍｇ／１ＢＡ＋０．５ｍｇ／１ＫＴ＋０．５ｍｇ／１ＺＴ＋０．５ｍｇ／ｌ生物素＋２％蔗糖等培养基上分化出了芽，在改良ＭＳ＋０．５ｍｐ／１ＩＢＡ＋０．５ｍｇ／１ＢＡ＋０．５ｍｇ／１ＫＴ＋０．５ｍｇ／１ＺＴ＋５％蔗糖＋１％麦芽糖等培养基上产生了胚状体。
In this paper, the effects of different media, such as medium, genotype, hormone ratio, sugar content and concentration, time of preparation, pretreatment temperature, inoculation method and organic additives on the induction frequency of callus were studied. Soybean anthers dedifferentiation start that there is a group effect, the appropriate drawing time is the late mononuclear. High concentrations of sucrose inhibited the production of somatic callus, whereas callus differentiation required lower sucrose concentrations. Callus was treated with B_5 +0.5 mg / 1 NAA + 1.0 mg / 1KT + 1% sucrose and modified MS + 0.1 mg / 1 IBA + 0.1 mg / 1 GA_3 + 0.4 mg / 1 NAA + 0.5 mg / 1 BA + 0.5 mg / 1KT + 0.5 mg / 1ZT + 0.5 mg / Buds were differentiated on medium such as sucrose and sucrose. Embryoid bodies were produced on media such as modified MS + 0.5mp / 1IBA + 0.5mg / 1BA + 0.5mg / 1KT + 0.5mg / 1ZT + 5% sucrose + 1% maltose.