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目的:运用基因芯片技术检测鼻息肉组织的基因表达谱,并通过分析基因的差异表达探讨相关基因在鼻息肉发病机制中的作用。方法:抽提6组鼻息肉和下鼻甲组织的总RNA,逆转录成cDNA,并分别用cy5和cy3标记制备成杂交探针,每组杂交探针与含有人类常见基因的BiostarH-40s基因芯片杂交,用扫描仪扫描芯片荧光信号图像,并用软件对扫描图像进行数字化处理和分析。结果:鼻息肉组织基因表达谱中差异表达的基因共1887条,其中上调基因1099条,下调基因788条;6张芯片共同的差异基因6条,其中上调基因4条,下调基因2条。在差异表达的基因中,主要包括编码横跨膜4超家族蛋白、IFN-γ诱导蛋白IP-30前体、补体因子前体、胰岛素生长因子结合蛋白的基因。结论:检测鼻息肉基因表达谱中差异表达基因为研究鼻息肉的发病机制提供了分子生物学的线索和方法,鼻息肉为多基因调控,其中IFN-γ诱导蛋白、胰岛素样生长因子结合蛋白等基因可能在鼻息肉发病中起重要作用。
OBJECTIVE: To detect the gene expression profile of nasal polyps using gene chip technique and to explore the role of related genes in the pathogenesis of nasal polyps by analyzing the differential expression of genes. Methods: The total RNA was extracted from 6 groups of nasal polyps and inferior turbinate tissues and then reverse transcribed into cDNA. The hybridization probes were prepared by cy5 and cy3 labeling. Each probe hybridized with Biostar H-40s microarray Hybridization, with a scanner to scan the chip fluorescence signal images, and software to scan images digitized and analyzed. RESULTS: There were 1887 differentially expressed genes in nasal polyps tissue, including 1099 up-regulated genes and 788 down-regulated genes. There were 6 differentially expressed genes in 6 chips, including 4 up-regulated genes and 2 down-regulated genes. Among the differentially expressed genes, genes encoding transmembrane 4 superfamily proteins, IFN-γ-inducible protein IP-30 precursors, complement factor precursors, and insulin growth factor binding proteins are mainly included. CONCLUSION: The detection of differentially expressed genes in nasal polyps gene expression provides clues and methods for studying the pathogenesis of nasal polyps. Polycystic nasal polyps are regulated by multiple genes, including IFN-γ-induced protein, insulin-like growth factor binding protein Genes may play an important role in the pathogenesis of nasal polyps.