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目的探讨病毒性乙型肝炎患者mIL 2R ,sIL 2R ,IL 6 ,IL 8和TNF α与乙肝发病的关系。方法采用A PAAP技术和ELISA法 ,检测92例慢性乙型肝炎患者mIL 2R ,sIL 2R ,IL 6 ,IL 8和TNF α的水平。结果慢性乙肝患者mIL 2R的表达显著低于正常对照组(P<0.05) ,在PHA激活后与正常人基本一致 ,但仍较PHA激活前显著增高(P<0.01) ;慢性乙肝患者血清sIL 2R ,IL 6 ,IL 8和TNF α明显高于正常对照组(P<0.01)。且在慢性活动性与非活动性肝炎之间 ,肝硬化活动期与稳定期之间 ,重型肝炎的肝坏死期与恢复期之间 ,血清TNF α ,IL 6和IL 8 ,3项指标的差异显著(均P<0.001)。结论病毒性肝炎患者血清细胞因子的高低 ,可在一定程度上反映肝细胞损害的程度 ,也提示上述细胞因子参与了乙型病毒性肝炎的发病过程。
Objective To investigate the relationship between mIL 2R, sIL 2R, IL 6, IL 8, TNFα and hepatitis B in patients with viral hepatitis B. Methods The levels of mIL 2R, sIL 2R, IL 6, IL 8 and TNFα in 92 patients with chronic hepatitis B were detected by A PAAP and ELISA. Results The expression of mIL 2R in chronic hepatitis B patients was significantly lower than that in normal controls (P <0.05), and was similar to that in normal subjects after PHA activation, but still significantly higher than that before PHA activation (P <0.01). Serum sIL 2R , IL 6, IL 8 and TNF α were significantly higher than those in normal control group (P <0.01). The differences of serum TNFα, IL-6 and IL-8, 3 between chronic active and inactive hepatitis, between active and stable cirrhosis, between hepatic necrosis and convalescence of severe hepatitis Significantly (all P <0.001). Conclusions The levels of serum cytokines in patients with viral hepatitis can reflect the degree of hepatocellular damage to a certain extent, and also suggest that these cytokines are involved in the pathogenesis of hepatitis B virus.