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目的探讨细胞因子诱导的杀伤(CIK)细胞联合顺铂(DDP)对胃癌耐顺铂细胞株SGC7901/DDP及亲代敏感细胞株SGC-7901的体外杀伤作用。方法体外培养SGC-7901/DDP及SGC-7901细胞,分为对照(A)组、DDP作用(B)组、CIK细胞作用(C)组和CIK细胞联合DDP(D)组。MTT法、RT-PCR法和Western blot法分别检测肿瘤细胞增殖、mdr-1基因mRNA表达和P-糖蛋白(P-gp)蛋白表达。结果与SGC-7901细胞相比,DDP对SGC-7901/DDP细胞的杀伤率明显降低(P<0.05),耐药指数为1.73。在SGC-7901/DDP细胞中,C、D组杀伤作用明显高于A组,且与SGC-7901细胞比较差异有统计学意义(P<0.05)。与B、C组相比,D组对SGC-7901/DDP细胞的体外杀伤活性明显升高,逆转倍数为1.41。C、D组SGC-7901/DDP细胞中mdr-1基因mRNA、P-gp蛋白表达均明显低于A组(P<0.05)。结论 CIK细胞与DDP的联合应用使SGC-7901/DDP细胞的生长受到明显抑制,其可能与CIK细胞下调mdr-1基因mRNA、P-gp蛋白表达及增加SGC-7901/DDP细胞对DDP的敏感性有关。
Objective To investigate the cytotoxicity of cytokine-induced killer (CIK) cells combined with cisplatin (DDP) on gastric cancer cisplatin cell line SGC7901 / DDP and the parental sensitive cell line SGC-7901 in vitro. Methods SGC-7901 / DDP and SGC-7901 cells were cultured in vitro and divided into control group (A), DDP group (B), CIK cell group (C) and CIK cells combined with DDP group (D). MTT assay, RT-PCR and Western blot were used to detect cell proliferation, mdr-1 mRNA expression and P-glycoprotein expression. Results Compared with SGC-7901 cells, the killing rate of DDP on SGC-7901 / DDP cells was significantly decreased (P <0.05), and the resistance index was 1.73. In SGC-7901 / DDP cells, the killing effects of group C and D were significantly higher than that of group A, and the difference was statistically significant (P <0.05) compared with SGC-7901 cells. Compared with group B and group C, the cytotoxicity of group D to SGC-7901 / DDP cells was significantly increased, with a reversal multiple of 1.41. The expressions of mdr-1 mRNA and P-gp protein in SGC-7901 / DDP cells in group C and D were significantly lower than those in group A (P <0.05). Conclusion The combination of CIK cells and DDP can significantly inhibit the growth of SGC-7901 / DDP cells, which may be associated with down-regulation of mRNA and P-gp expression of mdr-1 mRNA and increase of DDP sensitivity in SGC-7901 / DDP cells Sex related.