论文部分内容阅读
为寻找对柑桔绿霉菌具有抑制作用的活性化合物,采用生长速率法并结合光谱法评价两种商品化三唑类杀真菌剂和四种新型含氮化合物对柑桔绿霉菌的抑菌活性及与柑桔绿霉菌CYP51的结合能力。结果表明:7-甲氧基-2-氢-苯并[b][1,4]噻嗪-3-胺对柑桔绿霉菌表现出强抑制作用,EC50值达1.18mg/L,与商品化三唑类杀真菌剂烯唑醇的EC50值(0.09mg/L)接近;而其余三种新型含氮化合物显示出中等程度的抑制作用,EC50值分别为7.88、10.09和38.56mg/L。不同化合物与异源表达的重组柑桔绿霉菌CYP51的结合能力不同,对柑桔绿霉菌生长抑制的EC50越大,结合常数越大,即化合物与靶标酶CYP51的结合能力与抑菌活性呈正相关。
In order to find out the active compounds with inhibitory activity on citrus green mold, the growth rate method and spectrophotometry were used to evaluate the antibacterial activities of two commercially available triazole fungicides and four new nitrogen-containing compounds against Citrobacter citrulli. And citrus green mold CYP51 binding capacity. The results showed that 7-methoxy-2-hydro-benzo [b] [1,4] thiazin-3-amine showed strong inhibitory effect on Citrus green mold with EC50 value of 1.18mg / L, The EC50 values for the triazole fungicide diniconazole (0.09 mg / L) were close; while the three other novel nitrogen compounds showed moderate inhibition with EC50 values of 7.88, 10.09 and 38.56 mg / L, respectively. The binding ability of different compounds to heterologous expressed recombinant citrus green mold CYP51 was different. The greater the EC50 of citrus growth inhibition was, the larger the binding constant was. The binding ability of the compound to CYP51 was positively correlated with the antibacterial activity .