论文部分内容阅读
本研究以穗中红番木瓜品种为材料,比较系统地研究了通过实生苗顶芽、成龄植株侧芽直接产生丛生芽的微繁技术。芽增殖的初始培养物在MS~*+BA 0.5mg/l+NAA 0.2mg/l+3％蔗糖培养基上获得,MS~*+BA 0.5mg/l+NAA 0.1mg/l+ADS(腺嘌呤硫酸盐)40mg/l+GA_30.5～1.0mg/l+3％蔗糖与MS~*+KT 0.5mg/l+NAA 0.1mg/l+GA_3 0.5～1.0mg/l+3％蔗糖两种培养基交替继代,对幼芽长期保持较高的繁殖系数和正常化生长很有利,采用1/2 MS~*+KT 0.2mg/1+IBA 1.0mg/l+2％蔗糖+AC(活性炭)2 g/l培养基催根则获得较理想的效果。本研究为大量生产杂种一代(F~1)优质品种无性系和杂优单选品种无性系化提供了配套的繁殖技术,同时亦为试管苗的大田推广提供了理论依据。
In this study, the spike red papaya cultivars were used as materials to study the micropropagation technology that directly produced the clustered buds through the shoots of the terminal buds of the seedlings. The initial cultures of shoot proliferation were obtained on MS ~ * + BA 0.5 mg / l + NAA 0.2 mg / l + 3% sucrose medium with MS * BA 0.5 mg / l NAA 0.1 mg / Purine Sulfate) 40 mg / l + GA_30.5 to 1.0 mg / l + 3% sucrose and MS * KT 0.5 mg / l NAA 0.1 mg / l + GA_3 0.5 to 1.0 mg / l + 3% Alternate subculture of the medium, it is very beneficial to maintain the high reproductive coefficient and normal growth of shoots for a long time. The optimal conditions were as follows: 1/2 MS ~ * + KT 0.2mg / 1 + IBA 1.0mg / l + 2% sucrose + ) 2 g / l medium for a better result. This study provided a set of breeding techniques for the clonalization of clonal and hybrid varieties of high quality hybrid F1 generation (F ~ 1), and provided theoretical basis for the popularization of the test tube seedling.