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背景:适当强度的恒磁场可抑制血管平滑肌细胞增殖,可能用于抑制经皮冠状动脉介入治疗术后再狭窄。目的:观察不同磁感应强度恒磁场对培养的大鼠主动脉血管平滑肌细胞骨桥蛋白基因表达的影响,以探讨磁场是否能用于经皮冠状动脉介入治疗术后再狭窄的防治。设计:随机分组,对照观察。单位:解放军第四军医大学西京医院心内科。材料:实验于2006-02/12在解放军第四军医大学西京医院心内科实验室(全军心血管病研究所)完成。纯种雄性SD大鼠,体质量200～250g。方法:用含体积分数为0.1小牛血清的DMEM培养液体外培养大鼠主动脉血管平滑肌细胞,实验随机分为对照组,1Gs恒磁场组、5Gs恒磁场组、10Gs恒磁场组、50Gs恒磁场组,其中对照组不予磁场干预,其他各组分别给予磁场干预继续培养48h。应用反转录-聚合酶链反应及蛋白免疫印迹技术结合吸光度扫描分析,观察恒磁场对血管平滑肌细胞骨桥蛋白及骨桥蛋白mRNA表达的影响。主要观察指标:各组血管平滑肌细胞骨桥蛋白及骨桥蛋白mRNA的表达。结果:对照组细胞表达一定量的骨桥蛋白及骨桥蛋白基因,各恒磁场组均较对照组降低,差异显著(P<0.05)。不同磁场强度组间分析显示,恒磁场的刺激作用具有磁场强度依赖性,随磁场强度加大,抑制骨桥蛋白及骨桥蛋白mRNA表达作用增强。结论:适当强度的恒磁场能在基因水平上抑制血管平滑肌细胞骨桥蛋白的表达,磁场对经皮冠状动脉介入治疗术后的再狭窄可能具有防治作用。
Background: The constant intensity magnetic field can inhibit the proliferation of vascular smooth muscle cells and may be used to inhibit restenosis after percutaneous coronary intervention. OBJECTIVE: To observe the effects of different magnetic induction magnetic fields on osteopontin gene expression in rat aortic vascular smooth muscle cells (VSMCs) in order to investigate whether magnetic field can be used in the prevention and treatment of restenosis after percutaneous coronary intervention. Design: randomized, controlled observation. Unit: Xijing Hospital, Fourth Military Medical University. MATERIALS: Experiments were performed at Cardiovascular Laboratory, Xijing Hospital, Fourth Military Medical University, Fourth Military Medical University from February to December 2006 (PLA Cardiovascular Institute). Pure male SD rats, body weight 200 ~ 250g. Methods: Rat aortic vascular smooth muscle cells were cultured in vitro with DMEM containing 0.1% bovine serum. The experiment was randomly divided into control group, 1Gs constant magnetic field group, 5Gs constant magnetic field group, 10Gs constant magnetic field group, 50Gs constant magnetic field Group, the control group without magnetic field intervention, the other groups were given magnetic field intervention to continue training 48h. Reverse transcription - polymerase chain reaction and Western blotting combined with absorbance scanning analysis were used to observe the effect of constant magnetic field on osteopontin and osteopontin mRNA expression in vascular smooth muscle cells. MAIN OUTCOME MEASURES: Expression of osteopontin and osteopontin mRNA in vascular smooth muscle cells in each group. Results: The control group expressed a certain amount of osteopontin and osteopontin genes, and each constant magnetic field group decreased compared with the control group (P <0.05). The analysis of different magnetic field strength groups showed that the stimulating effect of constant magnetic field has the magnetic field intensity dependence, and as the magnetic field strength increases, the inhibition of osteopontin and osteopontin mRNA expression is enhanced. Conclusion: The proper intensity of constant magnetic field can inhibit the expression of osteopontin in vascular smooth muscle cells at gene level. The magnetic field may have a preventive and therapeutic effect on the restenosis after percutaneous coronary intervention.