【摘 要】
：
Shenfu injection (SFI),a Chinese medicinal product,shows potent efficacy in treating sepsis.The aim of the present study was to clarify the protective effects of SFI against lipopolysaccharide (LPS)-induced myocardial inflammation and apoptosis.Experiment
【机 构】
：
Cardiology Department, the First Affiliated Hospital with Nanjing Medical University, Nanjing 210029
论文部分内容阅读
Shenfu injection (SFI),a Chinese medicinal product,shows potent efficacy in treating sepsis.The aim of the present study was to clarify the protective effects of SFI against lipopolysaccharide (LPS)-induced myocardial inflammation and apoptosis.Experiments were carried out in Sprague-Dawley (SD) rats treated with LPS or LPS + SFI,and in H9C2 cardiomyocytes.The sepsisassociated myocardial inflammation and apoptosis was induced by the intraperitoneal injection of LPS (20 mg·kg-1).SFI attenuated the increased expression of tumor necrosis factor (TNF)-a and interleukin (IL)-1β induced by LPS both in serum and heart.In LPS group,cell viability was reduced,and reversed after SFI administration.LPS treatment increased the expression levels of cleaved-caspase 3 and Bax,and those of Bcl2 and Bcl2/Bax.These two trends were reversed by SFI administration.The expression levels of phosphorylated mitogen-activated protein kinase kinase (p-MEK) and phosphorylated extracellular regulated protein kinases (p-ERK) were increased by LPS,and reversed by SFI.MEK inhibitor U0126 attenuated the apoptosis induced by LPS.These results indicate that SFI could treat LPS-induced cardiac dysfunction.In conclusion,SFI attenuates the inflammation and apoptosis induced by LPS via downregulating the MEK and ERK signaling pathways.
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