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环子孢子蛋白(CSP)是疟疾疫苗研制中的一个候选抗原,而抗原中自然存在的多态性则可能影响疫苗的效果。为了确定CSP中B和T抗原决定簇多态性的程度及探讨抗原变异的机制,本文运用序列分析的手段,对地理间隔遥远的巴布亚新几内亚(PNG)和巴西两地的间日疟原虫人体分离物的CSP的基因序列及相对应的氨基酸序列进行了分析比较。结果显示:在一个分离株的不同克隆间,CSP的多态性是普遍存在的。CSP中重复区域的氨基酸序列可分为两种类型:Ⅰ型是GDRA(D/A)GQPA,Ⅱ型是ANGA(G/D)
Circumsporozoite protein (CSP) is a candidate antigen in the development of malaria vaccine, while naturally occurring polymorphisms in antigens may affect the vaccine’s efficacy. In order to determine the degree of polymorphism of B and T epitopes in CSP and to explore the mechanism of antigenic variation, this paper uses the sequence analysis method to analyze the genetic relationship between Plasmodium vivax in both geographically distant PNG and Brazil The CSP gene sequences and corresponding amino acid sequences of isolates were analyzed and compared. The results showed that polymorphisms of CSP were ubiquitous among different clones of one isolate. The amino acid sequence of the repeat region in CSP can be divided into two types: type I is GDRA (D / A) GQPA, type II is ANGA (G / D)