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从我国北方8个毛白杨根癌病发病苗圃分离到根癌土壤杆菌(Agrobacterium tumefactens)8株。经质粒型、生物型、寄主范围和对土壤杆菌素agrocin 84和D286的敏感性测定,证明5株系nopaline质粒型,其中生物Ⅰ型2株,生物Ⅱ型2株,Ⅰ—Ⅱ中间型1株,3株系agropine质粒型,其中生物Ⅰ型2株,生物Ⅱ型1株。所有分离菌株均系宽寄主群,其中1株经回接能侵染单子叶植物美人蕉(Canna indica),水仙(Narcissus)和吊兰(Chlorophytum)。分离菌株中,5株nopalinc质粒型菌株对土壤杆菌素84敏感,3株agropine质粒和3株生物Ⅰ型nopaline质粒菌株对土壤杆菌素D286敏感。在温室中,合并使用两种上壤杆菌素产生菌——放射土壤杆菌(A. radiobacter)K84和D286的菌体悬浮液,预浸毛白杨和向日葵幼苗根部或与致病的毛白杨根癌土壤杆菌共接种枝茎,降低根癌病诱发率达94%以上。表明放射土壤杆菌K84和D286可以控制毛白杨根癌病。
Eight Agrobacterium tumefactens strains were isolated from eight outbreaks of Populus tomentosa in North China. Five isolates of nopaline were identified as plasmid type, biotype, host range, and susceptibility to Agrobin 84 and D286. Among them, there were 2 biotype Ⅰ, 2 biotype Ⅱ and 1-Ⅱ intermediate 1 Three strains of agropine plasmid type, including biological type Ⅰ 2 strains, biological type Ⅱ 1 strain. All isolates belonged to a broad host group, of which one was able to infect monocotyledonous Canna indica, Narcissus and Chlorophytum. Among the isolates, 5 strains of nopalinc were sensitive to streptozotocin 84, 3 strains of agropine and 3 strains of strain nopaline were sensitive to streptozotocin D286. In the greenhouse, two suspension cultures of the streptozotocin-producing bacteria, Agrobacterium radiobacter K84 and D286, the roots of pre-soaked poplar and sunflower seedlings were used either in combination with pathogenic Populus tomentosa Agrobacterium were inoculated with stems and roots to reduce the incidence of root-knot disease induction rate of 94%. This indicated that Agrobacterium radiobacter K84 and D286 could control Populus tomentosa cancer.